FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-069_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-069_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24051999
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATAG1358090.5646474540432169No Hit
GCTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTC746380.31031932106765847No Hit
GGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATA494410.20555879783630457No Hit
GTTTTCTTTGCACTTTACCTTTATAACAAACCTTGGAATCAATTTACTTG412850.17164893446070742No Hit
CTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCGTTGAATTATTCCATG403530.16777399666447682No Hit
GGGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGAT383150.15930068847915718No Hit
GGCAAATCCGCCGAATTTAAGCATATAATTAAGCGGAAGAAAAGAAAATA350460.14570930258229264No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG346410.1440254508575358No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA328280.1364876158526366No Hit
GTTTGACTGGGGCGGTACATCTGTTAAATATTAACGCAGATGTCCAAAGA310320.12902046104359144No Hit
CTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCG300070.12475886099945373No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC292200.1214867836972719No Hit
AGGGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGA291090.12102528359493113No Hit
GTTGAATTATTCCATGAATGCCTTTAATTTGAGCAAAGTGAGCAGGAATG290580.12081324300736915No Hit
GTTCATTTTATTTTTTGTTTTGTAGCAATAGTAATTCGTTTTTATGAATT278910.11596125544492165No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCC278560.11581573739463402No Hit
CGGCGAGTGAACAAGGACCAGCTCAAACGGTTAATCCTTCGTTTGGTGCA273380.11366207025037711No Hit
GGAATGCTTCCCCATAGAGGGTGAAAGGCCCATAGTTCGTTTTAATTTAT272840.11343755668707618No Hit
GCAAGCCTTTCGACGGAATTGTCGTTGAATTATTCCATGAATGCCTTTAA265920.11056045694996079No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC249790.10385415366099093No Hit
CAAGGACCAGCTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGAC247220.10278563540602176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)