FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-058_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-058_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18305037
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATAG1117100.6102691843780486No Hit
GCTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTC796690.43522993152103434No Hit
GTTTTCTTTGCACTTTACCTTTATAACAAACCTTGGAATCAATTTACTTG441080.24096099887697575No Hit
GGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATA402320.21978649920237803No Hit
CTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCGTTGAATTATTCCATG394220.21536148766047292No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA350530.19149374022024648No Hit
GGCAAATCCGCCGAATTTAAGCATATAATTAAGCGGAAGAAAAGAAAATA315200.1721930417294431No Hit
CTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCG308240.16839080958973204No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC302320.16515672708009277No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC302290.16514033814845608No Hit
GGGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGAT291340.15915837810106584No Hit
CGGCGAGTGAACAAGGACCAGCTCAAACGGTTAATCCTTCGTTTGGTGCA280710.15335123332446693No Hit
CAAGGACCAGCTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGAC253790.13864489866914775No Hit
GCAAGCCTTTCGACGGAATTGTCGTTGAATTATTCCATGAATGCCTTTAA243540.1330453470266135No Hit
TTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATAGC230030.12566486481289277No Hit
GTTGAATTATTCCATGAATGCCTTTAATTTGAGCAAAGTGAGCAGGAATG228200.12466513998305494No Hit
AGGGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGA224970.12290059834350513No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG224930.12287874643465621No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCC223010.12182985480990834No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT220890.12067170364091588No Hit
GGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCGTTGAATT220040.12020735057787647No Hit
GTTTGACTGGGGCGGTACATCTGTTAAATATTAACGCAGATGTCCAAAGA218130.11916392193034081No Hit
GGAGTCTAGCAAATGTGCAAGTGTATATGATTCTTTAAACATTTCTCTTT215390.1176670661741902No Hit
GTTCATTTTATTTTTTGTTTTGTAGCAATAGTAATTCGTTTTTATGAATT214330.11708799058969398No Hit
CGGAATTGTCGTTGAATTATTCCATGAATGCCTTTAATTTGAGCAAAGTG213070.11639965546095317No Hit
GTCTACTTGACATTTTTATTATAAGGATAACTACGGAAAAGCTGTAGCTA210870.1151978004742629No Hit
ATTTACTTGGAGAAGAGGTTCGTTGAACTCAATTCAAAAAATTTCTTGAA204000.1114447351294619No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATTT201390.11001889807707027No Hit
GTGCAAGTGTATATGATTCTTTAAACATTTCTCTTTTTAATATACGCATA191310.10451221704714392No Hit
GAAATATATTATGCTTTATCCTTCGGGAAGGCATTCTGATATTATTTGAA185100.10111970819835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)