FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-055_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-055_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55008920
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1652520.30040946086561965No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG1296940.23576903527646062No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1024820.18630069450554565No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA1021040.18561353322333907No Hit
GTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATGGCGCTGG993880.18067615215859537No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG930110.16908348682359153No Hit
GGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAG877430.15950685816045834No Hit
GTTGGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCT832930.15141726105511616No Hit
GTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTG832220.15128819107882868No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA829820.15085189820123718No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT816600.14844865160050408No Hit
GTAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAA780960.14196970236827047No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG752620.13681781063871096No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGC751060.1365342202682765No Hit
GGGGAATTAGAGTTCGATTCCGGAGAGGGAGCCTGAGAAATAGCTACCAC745040.13543985230031785No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG742190.13492175450817795No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAA730490.13279482672991944No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT705050.12817012222744967No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG693540.12607773430200048No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC688350.12513425095420888No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT686130.12473068004243676No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA656180.11928610850749297No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT650000.11816265434769488No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA649440.11806085267625688No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT645400.1173264263323112No Hit
GTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA624590.11354340350619499No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT608000.11052752898984383No Hit
CCGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAA602550.10953678058031316No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG601580.1093604455422866No Hit
GGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATA595840.10831697841004696No Hit
CTGAGAAATAGCTACCACATCTAAGGAAGGCAGCAGGCGCGTAAATTACC579740.10539018035620405No Hit
GGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGA573230.10420673592573713No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA566440.10297239065955122No Hit
AGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGAT553530.100625498555507No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC553240.10057277983279803No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)