FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-048_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-048_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19897957
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG1442070.724732694919383No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG944960.47490302647653726No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA886620.4455834335153101No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA674730.339095114136592No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT649660.32649583070261934No Hit
CCCAATTCTAAAGAAGAGAGGTAGTGACAAGAAATAACAATACAAGGCCA618310.3107404443581821No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT582330.29265818596351373No Hit
AGAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAA571210.2870696725296974No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG562860.28287326181275796No Hit
CGCACAATTAATGTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAA529190.26595192662241657No Hit
TATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAGTGGCAC526200.26444925979084183No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT498860.25070915571885094No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT489740.2461257706004692No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA462550.23246105115213586No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA457260.22980248675781137No Hit
CGGGTATTCATATTAATTGAATTAATAAGTGGCACTTCTCATAGTGTTTC449510.22590761453550232No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA448700.2255005375677513No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG439190.2207211524278598No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA434000.21811284444930704No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT422870.2125193053739135No Hit
GTAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAA420220.21118751035596267No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT417020.20957930505126734No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA403370.20271930429842622No Hit
AATGGATCCGTCCATTTTTAGTGTGTATCAATCGAGTTTCTGACCTATCA393750.1978846371011858No Hit
GTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA386000.19398976487887676No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT379370.19065776451321106No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA365660.18376760991090693No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA359580.18071201983198576No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG359070.18045571211154993No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC354330.17807355800396996No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC353060.177435301523669No Hit
GAACTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAA351770.17678699376021367No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA333180.1674443260682491No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC329880.16578586434778203No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG325000.16333335125812162No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT318420.16002647910034182No Hit
CTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTTATTAAAATTA311760.1566794018099446No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA308780.15518176061994707No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA307810.1546942733869613No Hit
CTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAGTGGCACTTCTC302340.15194524744424767No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG294410.1479599136735495No Hit
AGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGAATAACA293060.14728145206063115No Hit
GGGAAATATATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATA293060.14728145206063115No Hit
GAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAG291680.14658791352298128No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT289850.14566822111435862No Hit
GTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCG286590.14402986196020023No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG278840.14013498973789118No Hit
GCACAATTAATGTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAAC277080.13925047682030875No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG275760.1385870921321219No Hit
GGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCGTAGTTTGTTGCTTTCTAT275710.13856196392423606No Hit
GAAATATATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAG275570.13849160494215562No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT272780.13708945094212435No Hit
CTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAG271510.1364511944618234No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA269820.13560186103528116No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC268240.13480780966608782No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA267970.13467211734350418No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC263820.13258647608897736No Hit
GTTTTACTTATCTATTGATGGGGAAATATATTACTCGGTTCGCCGGGTAT261380.13136021954414717No Hit
TAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAAA257950.12963642448317686No Hit
AGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAG250870.1260782702465384No Hit
CTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACG250850.12606821896338402No Hit
GTTACCTTCTATACGCACAATTAATGTAATATGTTAATTATTGTAGAACT247590.12442985980922565No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG239990.12061037221057418No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA238000.11961026953671676No Hit
CCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTG236940.11907755152953642No Hit
GACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGG236670.11894185920695276No Hit
GAAAGCTCTCCCTAAGAGGGTGAAAGGCCCGTAGTTTGTTGCTTTCTATT236410.11881119252594625No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA234760.11798196166571272No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTG229950.1155646280670925No Hit
GAACTATCCTTGAAACAAGGAGGGAACAGCAATTAAAGGGGGATGATTTA228990.11508216647568392No Hit
CTTAGATGAACTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGTT225920.1135392945114918No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAAACTGAAATATGTT225410.113282986791056No Hit
CGTAGTTTGTTGCTTTCTATTTTTTGGAATTTTACTTCTAGAGTCGTGTT224420.11278544827491585No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA223550.11234821745770181No Hit
CTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGAATTATCCG223540.11234319181612465No Hit
GCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACGACAGTGAAATACC223260.11220247385196379No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT221580.11135816606699875No Hit
CTTGAGTGTGTACTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAG221470.11130288400964983No Hit
GTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGATGAACTAGGCTGCAC218050.10958411459025667No Hit
CACTGATATGTATAACGAGTTATTAAAATTACGATTCAGCTTGCTGTTTC217550.10933283251139803No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT215150.10812667853287651No Hit
GTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAACTTCCTGTCGGC215090.10809652468341349No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG214430.10776483233932005No Hit
CGGCGAGTGAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCA213620.10735775537156905No Hit
GCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAA212260.10667426811707353No Hit
AGCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACGACAGTGAAATAC212110.10659888349341592No Hit
CTGATATGTATAACGAGTTATTAAAATTACGATTCAGCTTGCTGTTTCGT211730.10640790911348336No Hit
GGACATTAGAATAGAGAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGA211470.10627724243247687No Hit
GGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTA211420.10625211422459101No Hit
CTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTT210140.10560883210271287No Hit
GTAGAACTGGCTGGAAGGCAACTTCCTGTCGGCGCATACAATACCGGTAA208040.10455344737150653No Hit
AGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCGTAGTT207280.10417149861164138No Hit
GGCAACTTCCTGTCGGCGCATACAATACCGGTAAGCAATTACGCTATATT207000.10403078064748054No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT206180.10361867803815236No Hit
GGAGACATAGCCAGGTGGGGAGTTTGACTGGGGCGGTACATCTGTTAAAC205280.10316637029620679No Hit
CCTTAGATGAACTAGGCTGCACGCGTGCTACACTGATATGTATAACGAGT204850.10295026770838836No Hit
GGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTT204550.10279949846107317No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT204380.10271406255426123No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG204280.10266380613848948No Hit
CTTGGGGGCATTTGTATTCAGATGTCAGAGGTGAAATTCTTAGATTTTCT203820.10243262662593952No Hit
GAGCCATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAG202720.1018798060524505No Hit
TGGAGACATAGCCAGGTGGGGAGTTTGACTGGGGCGGTACATCTGTTAAA202070.10155313934993428No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC201200.1011159085327202No Hit
GTCTAACACAAGGAAGTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGA199050.10003539559362802No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)