FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-046_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-046_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20462167
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA1983120.9691642141323545No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC1280720.6258965631548213No Hit
GTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATAG991300.4844550433001548No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC906130.44283188579195937No Hit
GACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTC833330.40725403130567744No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT810790.3962385802051171No Hit
GGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTG610770.2984874475904727No Hit
GAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAG546550.26710269738293113No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG534850.2613848279119215No Hit
AATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGT472710.2310165878325595No Hit
AACCAGTGTTGCGAAGCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTA468230.22882718140263442No Hit
GTGACAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTG466980.22821629791214196No Hit
GTTGAACTCAATTCAAAAAATTTCTTGAACGGGGTTTTCGGATTCAGTTC428110.20922026489178785No Hit
AGCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATG413640.2021486776058469No Hit
GTTCGTTGAACTCAATTCAAAAAATTTCTTGAACGGGGTTTTCGGATTCA406080.19845405425534843No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG405580.1982097008591514No Hit
CAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTG393540.1923256710787279No Hit
CAAACCAGTGTTGCGAAGCTAAGTCTGTTGGATAATGGCTGAACGCCTCT390860.191015936875112No Hit
ATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTACAGCAA388510.18986747591298617No Hit
GTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAAC385950.18861638652445756No Hit
CGAAGCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACC355290.17363263626965805No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA353310.17266499682071795No Hit
AGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCA350430.17125752125862329No Hit
AAACCAGTGTTGCGAAGCTAAGTCTGTTGGATAATGGCTGAACGCCTCTT346650.16941020958337402No Hit
GTTTTCTTTGCACTTTACCTTTATAACAAACCTTGGAATCAATTTACTTG343310.16777792889677814No Hit
CCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATT338410.16538326561404762No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGTTAAAGCATTTAC337660.16501673551975213No Hit
GAGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACC330200.16137098284849302No Hit
GTTCTACTAATAATTTGTTTTTTACTCTATTTCTCTCTTCTTTTAAGAAT326690.15965562200719016No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATTT325580.15911315746763283No Hit
CAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGTTGGATAAT323110.15790605169041969No Hit
GTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCT320990.15686999329054446No Hit
CGAGTTTTTAAAAATATGCTTATATTTGTATCTTTGATGCTTATATTTTG315700.15428473435878026No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTATTGCAACT305150.14912887769902378No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA282200.13791305681358187No Hit
CAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTG278650.13617814770058323No Hit
GTTTAAGGCAACAACAGGTCTGTGATGTCCTTAGATGAACTAGGCTGCAC277000.13537178149313317No Hit
GTTTTTTACTCTATTTCTCTCTTCTTTTAAGAATGTACTTGCTTGATTGA267490.13072417989746637No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC266730.13035276273524696No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC265980.12998623264095147No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG263010.12853477346754133No Hit
GAACTAGGCTGCACGCGTGCTACACTGATATATATAACGAGTTTTTAAAA259350.12674610660737937No Hit
GCTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTC259010.1265799462979654No Hit
ATTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTT252630.12346199696249181No Hit
GCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGTTGGATAATGG248680.12153160513253558No Hit
GTTCAAAGCAAACAGTTAAAGCATTTACTGTGTTTGAATACTATAGCATG240550.1175584189103725No Hit
ATTTACTTGGAGAAGAGGTTCGTTGAACTCAATTCAAAAAATTTCTTGAA240230.11740203273680641No Hit
CTTGGTTCCTACAGCGATGAAGGCCGCAGCAAAATGCGATACGCAATGAA239360.11697685782742365No Hit
CTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCGTTGAATTATTCCATG238600.11660544066520423No Hit
ATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGTT238020.11632199072561572No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT229980.11239278811476809No Hit
AGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCG229860.11233414329968082No Hit
GGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATA222560.10876658371520476No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT222530.10875192251143292No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATTTC219730.10738354349272977No Hit
ATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTT217140.10611779290042936No Hit
TTTGTTTTTTACTCTATTTCTCTCTTCTTTTAAGAATGTACTTGCTTGAT216070.1055948766325678No Hit
GTTCATTTTATTTTTTGTTTTGTAGCAATAGTAATTCGTTTTTATGAATT210570.1029069892744009No Hit
GGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGC209930.10259421692726874No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA209550.10240850834615904No Hit
GTTGAATTATTCCATGAATGCCTTTAATTTGAGCAAAGTGAGCAGGAATG208910.10209573599902688No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)