FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-038_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-038_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29963269
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCG1546360.5160852108626732No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA1214050.40517942151105074No Hit
GTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGG755290.25207196184101277No Hit
GGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCC701040.23396646073564267No Hit
CCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGT655620.21880790110051077No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT619300.20668639326369898No Hit
GAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGT606090.2022776620267969No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG542510.18105834847325905No Hit
GAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCC537570.17940966321131382No Hit
GTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGG531600.17741722373483346No Hit
GCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATAT530220.17695665983574754No Hit
AGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGAT508690.16977119552609563No Hit
CATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCC502420.1676786334628575No Hit
GGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGG479950.16017945171469775No Hit
CTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAG451820.15079129049637408No Hit
GAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAG445780.14877548908298358No Hit
CCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATATAGAC428190.14290496807941752No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA424380.14163341122759335No Hit
CCGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAA421420.14064553503824967No Hit
CCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTC399470.13331989910713682No Hit
TGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGG392030.1308368589555432No Hit
GGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATA390230.13023612343499635No Hit
AAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCG369570.12334101462694207No Hit
GGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGA367280.12257674554802414No Hit
GGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAG354150.11819471366759081No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG350080.11683638390724324No Hit
CCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGGGTCCGCCG345820.11541464317528238No Hit
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG342760.11439339279035274No Hit
GAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCC334390.11159997261980995No Hit
ATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGG331710.11070554417810688No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA327020.10914029440512649No Hit
AATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCG317850.1060798806698962No Hit
TGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCC314590.10499188189379469No Hit
GAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCG303990.10145421716168553No Hit
AGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAG303180.10118388617743947No Hit
GTTGGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCT299970.10011257449913091No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)