FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-027_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-027_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34205018
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCG962470.28138269069175753No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA704600.2059931674352576No Hit
GGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCC584950.17101291980024685No Hit
GTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGG555640.16244400163742057No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT539080.15760260672863846No Hit
GAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGT495080.14473899706762325No Hit
GCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATAT462970.13535148556273235No Hit
GTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGG416450.12175114189385897No Hit
CATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCC387460.11327577725584005No Hit
GAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCC380130.11113281682822093No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG372810.10899277994825204No Hit
CTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAG349010.10203473654070289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)