FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-024_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-024_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20083710
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA3014621.5010274496096587No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG2516961.2532345866376282No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG1479750.7367911605973199No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG1141750.5684955618259774No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA1102310.5488577558628361No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC1076200.5358571698157362No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC887940.44211950879593465No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC864990.43069233722255496No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT754300.3755780182048038No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA684190.3406691293590676No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA631790.31457833238978256No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC620260.3088373612245945No Hit
GTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGTTACCTTCTAT617580.30750294641776843No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA588800.29317292472356954No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT563450.2805507548157188No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC562840.28024702607237406No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA507280.25258281462936877No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA504840.2513678996559898No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT502360.2501330680437031No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTGCCTTCTAT501180.2495455271959215No Hit
GTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTT497240.24758373826349814No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC481010.2395025620266375No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA471350.23469269373039148No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT467330.2326910715201524No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT465940.23199896831810457No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG445640.2218912740723701No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG433060.21562749113585086No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT425000.2116142883959189No Hit
TGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTG419220.20873633407373438No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG407340.20282109231810258No Hit
CCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCAT400240.199285888911959No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC398090.198215369570662No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA394980.19666685089557656No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC394830.19659216349967212No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA384010.19120471267509836No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA379980.18919811130513237No Hit
CGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATT375520.18697740606690696No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT357430.17797010612083125No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT355600.17705891989079708No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA353170.1758489840771451No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG346020.1722888848723667No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG343080.17082501191263963No Hit
TCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCAT342230.17040178333584782No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT328310.1634707929959156No Hit
TTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGA324980.16181273280683697No Hit
TTTCGAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCA319030.1588501327692941No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGC318670.15867088301912347No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG316690.15768500939318483No Hit
TTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGG316410.15754559292082987No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT310040.15437386817475457No Hit
CGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTCGTTCTT304960.15184445503345748No Hit
CCTTCACATAGGTCTTACACTCGGGGTGCGTTTTCTTTGCACTTTACCTC301690.15021626980274064No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA297910.1483341474259487No Hit
TGTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGTTACCTTCTA295310.14703956589693837No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC294170.1464719416880646No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACAC293820.1462976710976209No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG292010.14539644318704065No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG289810.14430102804710881No Hit
GTTGAATTTCAAAGAATCGACATTTTAAGCAACGCTTCTAGCTTAATCCA285790.14229940583686979No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT283840.14132846969011204No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA283530.1411741157385762No Hit
TGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAG280980.13990443000820066No Hit
CTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATT273040.13595097718499222No Hit
GTTGAATTTCAAAGAATCGATATTTTAAGCAACACTTCTAGCTTAATCCA270760.1348157287672447No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC258630.12877600801843883No Hit
CTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGC258370.1286465498655378No Hit
TGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAA257960.12844240431673232No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT251380.1251661172163908No Hit
CTTACACTCGGGGTGCGTTTTCTTTGCACTTTACCTCTATAGCAAACCTT250680.12481757603550339No Hit
GGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTT250130.12454372225052045No Hit
TCATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCT246740.12285578710308005No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA245840.12240766272765341No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA241380.120186957489428No Hit
TGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAG241040.12001766605871127No Hit
GTGTGTATGAAATGTTAAACATTTGGTACTTCGTTACCTTCTATACGCAC240580.11978862471127098No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG240130.11956456252355765No Hit
AGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAAT238970.11898697999522996No Hit
TTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTTG238120.11856375141843813No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG236280.11764758602867698No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG234820.11692062870854039No Hit
GCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGAATTC231320.11517792280410344No Hit
ATTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTT230910.11497377725529795No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA230380.11470988178976893No Hit
GGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTG229840.11444100716451293No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT226620.11283771773243091No Hit
GCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCTC224730.11189665654403494No Hit
TTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTG223770.1114186572102465No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT222490.11078132476519528No Hit
CTTTTACTGCTGATGTAAAAGAGAAATTATACGTGCTTCCTCTACGGATG216670.10788345380410294No Hit
GCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCTG210900.1050104786416454No Hit
GCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTC209570.10434825039795934No Hit
TTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGGAAAGCTCTCC208150.10364120971673063No Hit
GCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAG207660.10339723089010945No Hit
CCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACATATATACAA205970.10255575289625272No Hit
ATTCTTTTTAAGGACTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTT205220.10218231591673052No Hit
CTTTCTTTGCTTCGGCTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGT203080.1011167757351605No Hit
TGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTGCCTTCTA202620.1008877343877202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)