FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-014_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-014_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14725409
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG1508401.0243518533169436No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA718470.48791174493014083No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT573010.38913010837254164No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA510800.34688340405349694No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG504260.3424421012686303No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC465570.3161677886162618No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC436620.296507893261233No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT420330.2854453821961753No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT393670.26734062191413493No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC383420.26037986449136996No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA353620.2401427355939655No Hit
CTCAAAAGGAAAATCCCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCA345030.2343092813245459No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC315330.21414006225565618No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA287540.1952679209113988No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA277580.1885041019913267No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA275100.18681993824416016No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC268000.18199834041961077No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC262230.17807994331430793No Hit
GATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGT257060.17456900518009383No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA255220.17331946433542186No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG238500.1619649410077506No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA237270.16112965011701882No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA233930.15886146184462516No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT226290.15367315094609596No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG224260.15229458142724592No Hit
GACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTC220170.1495170694409914No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT210690.14307921769779025No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG210350.14284832428084002No Hit
TGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTA206260.14007081229458548No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT203020.1378705338507066No Hit
TTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTTG202540.1375445666738357No Hit
TCATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCT201630.1369265872343512No Hit
GGAAAATCCCTTGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG194650.13218648120401955No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC190960.12968060853182414No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA189720.12883852665824086No Hit
TGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAA188470.1279896538018061No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT185890.1262375802261248No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG181410.12319521990866264No Hit
TGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTG177290.1203973349738537No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG167780.11393911028209812No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA167060.11345015951679169No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAAATATGCATTTCAT164340.11160301218118968No Hit
CTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTGGTAATGCAAATTAT162490.11034668035366624No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG162160.11012257791956748No Hit
ATTTACTATTATTTTCTTATACATATAGTATTGTGACAGTAATCCAACTT160870.1092465411317268No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC160370.10890699198915291No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT158200.10743334871038217No Hit
AGAGGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCA156800.10648261111117524No Hit
CAGCAATTGGTTGACAAACCAGTGTTGCGAAGCTAAGTCTGTTGGATAAT152760.10373905403917813No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA152590.103623607330703No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA151880.10314144754824807No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG151710.10302600083977294No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA151400.10281548037137712No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAAATATGCATTTCATCCCCGTCTC150780.10239443943458547No Hit
CTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTT150170.10198018948064531No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT149540.1015523575610022No Hit
TGTGACAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATT148990.10117885350417093No Hit
GGATTAGTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTG148790.10104303384714135No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA147900.10043863637335983No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA147890.10043184539050834No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)