FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-009_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-009_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19369120
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA1789800.9240481756527916No Hit
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG1514250.7817856464310201No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG1431080.7388461633775825No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG1123860.5802328655096359No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT605690.3127090957152416No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC604260.311970807140438No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA569510.29402987848699375No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA557990.2880822670312332No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT470290.24280400968138974No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC434140.22414028102464129No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT432160.22311803530568244No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG404180.2086723609539308No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA401830.20745908951981298No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA393140.20297256664216032No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT387160.19988517805661796No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT385050.19879581519449516No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG371860.19198600659193601No Hit
GTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTAT370990.19153683801845411No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC354190.18286323797880336No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC353760.18264123512064565No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT346830.1790633751042897No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC339700.1753822579446046No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC335730.17333260364952047No Hit
GAAATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATG330440.17060145220846376No Hit
GTTAAACATTTGGTACTTCGTTACCTTCTATACGCACAATTAATGTAATA330250.17050335792230106No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG329830.1702865179213098No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC325550.16807681505406544No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG324640.1676069950519177No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA322460.16648149218962968No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA321180.16582064647232295No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC319060.1647261207530337No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA315440.16285716645877563No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT309180.15962521787257242No Hit
GTAAAAGAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTA292900.15122008640557752No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC290950.1502133292581181No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG289680.1495576463979778No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG286950.14814818639153457No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTATT279780.14444641780318362No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA277350.14319184351173414No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT274620.14178238350529088No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA270150.1394745863518838No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA266260.13746623491413135No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA259900.13418265775626356No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG255250.13178192917386025No Hit
AGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTT252810.13052219202524432No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT251270.12972711202160966No Hit
TCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCAT249660.1288958920178098No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA235840.12176082341376376No Hit
GAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGT233100.12034620055015406No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA230750.11913292911603623No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT230360.11893157768654436No Hit
CAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCA229910.1186992491140537No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG229720.11860115482789099No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT225090.11621075195982057No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT224360.1158338633866691No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA222730.11499231766853632No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA222720.11498715481136985No Hit
CGCACAATTAATGTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAA220050.11360867194792537No Hit
GGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTA219410.113278249089272No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT219280.11321113194610803No Hit
GAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCTTT216630.11184297479699647No Hit
TGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAG214350.11066584336304386No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG211920.10941126907159437No Hit
TCATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCT211220.10904986906994227No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTGGGAA210870.1088691690691162No Hit
CTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCC204780.1057249890547428No Hit
TTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTGGA202930.10476986047894793No Hit
TGCTGATTAAACAATACTATCTCGTTCTTTTTTGTTATCCTTTTACTGCT201490.10402640904697788No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC200260.10339137761550343No Hit
CAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTGTTTGGAA199980.10324681761484258No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTATTTGGGGTTTCGGCCTTGA198600.10253434332587129No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA197270.10184768332273225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)