FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-004_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-004_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33915902
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATAG1116220.32911405393257714No Hit
GCTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTC921560.27171914814472575No Hit
GTAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATGGCGCTAA757210.22326105317794584No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG733830.21636753166700387No Hit
GGCAAATCCGCCGAATTTAAGCATATAATTAAGCGGAAGAAAAGAAAATA526750.1553106268558035No Hit
GTTTGACTGGGGCGGTACATCTGTTAAATATTAACGCAGATGTCCAAAGA507570.1496554625025158No Hit
GGAATGCTTCCCCATAGAGGGTGAAAGGCCCATAGTTCGTTTTAATTTAT470830.1388227858424641No Hit
CTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCGTTGAATTATTCCATG463420.13663797000003125No Hit
GGACTATCCTTGAAAAAAGGAGGGAACGGCTTTGTAAACTTGGTTTCTTC463150.1365583613256106No Hit
GGACATTAGAAAAGAGAAGAAAAAAAGAGGGTCGAAAATAAAATTGCAGT461510.13607481234024088No Hit
GTTTTCTTTGCACTTTACCTTTATAACAAACCTTGGAATCAATTTACTTG458220.1351047659000784No Hit
GGAGTCTAGCAAATGTGCAAGTGTATATGATTCTTTAAACATTTCTCTTT457610.1349249092652762No Hit
GTTGAATTATTCCATGAATGCCTTTAATTTGAGCAAAGTGAGCAGGAATG442830.13056707145810248No Hit
CCCAATTCTAAAGAAGAGAGGTAGTGACAAGAAATAACAATGCAAGGCCA424150.12505933057596405No Hit
GAAATATATTATGCTTTATCCTTCGGGAAGGCATTCTGATATTATTTGAA419410.12366175606946853No Hit
GGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATA412790.12170986931145161No Hit
CTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTCG374810.11051158244294963No Hit
CAAAGAAGTAACACGTAATAAATTTATTTTATTTAGTGTGTATCAATCGA369680.10899901762895765No Hit
GGGGAATTAGAGTTCGATTCCGGAGAGGGAGCCTGAGAAATAGCTACCAC368000.10850367476589594No Hit
GAAAAAAGTTATTAACTTAAGGAATTATAACAAAGAAGTAACACGTAATA367260.10828548802859497No Hit
GGAGTGTGTAACAACTCACCTACCGAATGAACTAGCCCTGAAAATGGATG356740.10518369819561338No Hit
GGGAAATATATTATGCTTTATCCTTCGGGAAGGCATTCTGATATTATTTG354530.1045320864531334No Hit
GTTCATTTCAATCACGTTTAGGGGCGAAAGACTAATCGAAAAGCCTATTA341060.10056049814037085No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA339990.10024501191211131No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)