FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_MHC-003_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_MHC-003_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19138755
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATAG1009750.5275944020392132No Hit
GGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGATA370060.19335635990951344No Hit
GCTCAAACGGTTAATCCTTCGTTTGGTGCAAGCCTTTCGACGGAATTGTC304330.15901243314938718No Hit
GTTTTCTTTGCACTTTACCTTTATAACAAACCTTGGAATCAATTTACTTG285610.14923123264810068No Hit
GGGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGAT284070.14842658260686237No Hit
GCTCAAACGGTTAATCCTTCGTTTGGTGCAAACCTTTCGACGGAATTGTC251060.1311788567229164No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA238220.12446995637908527No Hit
AGGGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCCTAAGGGA226010.11809023105212435No Hit
GGGAAATCGTTTTCCTTGACCTATTCTCAAACTCCCAATAGGTAAAAAGG222930.11648093096964772No Hit
GTTTGACTGGGGCGGTACATCTGTTAAATATTAACGCAGATGTCCAAAGA222490.11625103095786533No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGAACAAGAGTTAGCCGCTCC207270.10829858055030227No Hit
GTTGAATTATTCCATGAATGCCTTTAATTTGAGCAAAGTGAGCAGGAATG205640.1074469055066539No Hit
CAGTAATCCAACTTGGTACGAGAGGATTAGTTGGTTCAGACAATTGGTAC195960.10238910524744164No Hit
GTTCATTTTATTTTTTGTTTTGTAGCAATAGTAATTCGTTTTTATGAATT193240.10096790517460513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)