FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_KHC-012_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_KHC-012_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13819204
Sequences flagged as poor quality0
Sequence length101
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT902910.6533733780903734No Hit
GGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCG397110.2873609796917391No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT386760.27987140214443607No Hit
CGGAACGATACGGCAGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGG368260.2664842345478075No Hit
ATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTC338940.2452673829838535No Hit
CCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGCCCC323290.23394256282778658No Hit
GTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCG317930.23006390237816882No Hit
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAG286250.20713928240729348No Hit
GGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCC265720.19228314452844028No Hit
GCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGG250950.1815951193715644No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCC227750.16480688757471126No Hit
GAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATC227160.16437994547298093No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG220590.15962569189947554No Hit
GAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGT216150.15641277167628467No Hit
GGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGT209250.15141971997808268No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA200100.14479849924785826No Hit
GAACGATACGGCAGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTC180680.13074559142480277No Hit
CTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAG174610.1263531531917468No Hit
GTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTG155210.11231471798230926No Hit
GAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCG152360.11025237054174755No Hit
GTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGG145490.10528102776397252No Hit
GCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGG145450.10525208253673655No Hit
GGGGGGGCGAGCCCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC144600.10463699645797254No Hit
CGATACGGCAGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC140110.10138789470073674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)