FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_KHC-008_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_KHC-008_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27820450
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG2223640.7992825421587357No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA2066200.7426910779660286No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG1664640.598351212866794No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG1661330.597161440594958No Hit
CTGAAATATGTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTT935580.3362921879408852No Hit
GTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATA882660.3172702095041597No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC856900.30801083375718225No Hit
AGAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAA849490.3053473254386611No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT810290.29125697104108667No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT785930.28250082223687967No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA771440.27729242337920484No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT747050.26852549114050994No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA688780.24758046688676855No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT688290.24740433745679885No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA678320.24382064272864024No Hit
GGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACT667800.2400392517015361No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA650890.2339609891285008No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA634100.22792586029341724No Hit
CGGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTG608890.2188641808453853No Hit
CAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTG584440.21007568173771451No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC567090.20383926212552278No Hit
GGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAG553180.19883934300128142No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC545550.1960967561631821No Hit
GGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAAC543590.1953922384433034No Hit
CTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATC539090.19377472327011244No Hit
CGAAAGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAA537100.19305942211574578No Hit
GTTTGACTGGGGCGGTACATCTGTTAAACAATAACGCAGATGTCCAAAGA535840.19260651786725233No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGCTACCTTCTAT520990.1872687177957222No Hit
AATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTAT519880.18686973071966845No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT513550.1845944260427132No Hit
GTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTTGATAT502380.180579393935037No Hit
GAAGACCCTGTTGAGCTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAG500940.1800617890796159No Hit
GAGAAATTATACGTGCTTCCTCTACGGATGCATTCATAATCCTATCACCT499130.17941118853217689No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG492970.1771969899839866No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG486740.1749576300886578No Hit
CAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATG477210.17153209239965567No Hit
GTTTTGTAAGAAATATGCATTTCATCCCCGTCTCGAAAGGGAAACAGGTT469410.16872839943279136No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC467410.16800950380026203No Hit
TATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAGTGGCAC460400.16548977460824682No Hit
GGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGAT454250.1632791705382192No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA452480.16264294790343076No Hit
GTGGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCC450680.16199594183415436No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTT435890.15667970863160013No Hit
CGGCGAGTGAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCA429710.15445832112708457No Hit
GTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGCATTGCG425700.15301693538386332No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTTTTT422630.1519134305879308No Hit
CAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCA420180.1510327834380824No Hit
GTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGAGATGGTAACA418990.1506050405367275No Hit
GAAATATATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATAAG418810.15054033992979984No Hit
TTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCACA415020.1491780327061568No Hit
AAGGAGTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTA413620.1486748057633863No Hit
CGCACAATTAATGTAATATGTTAATTATTGTAGAACTGGCTGGAAGGCAA413470.1486208885909466No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG412930.14842678677016366No Hit
GTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGAGCAGG395170.14204299355330344No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA390710.14043985629276307No Hit
GTTTTTACGACAGTGAAATACCACTACTTTTAAAGTTGTTTTACTTATCT387260.13919976132665No Hit
GTGAAATACCACTACTTTTAAAGTTGTTTTACTTATCTATTGATGGGGAA384720.13828676387333777No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA375760.13506611143960648No Hit
GTTTTACTTATCTATTGATGGGGAAATATATTACTCGGTTCGCCGGGTAT374920.13476417527394416No Hit
CTTGGAAGTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAG372820.1340093348597884No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAAACTGAAATATGTT370500.1331754159260544No Hit
GTCTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCG368260.13237025281762155No Hit
GCTAAGTCTGTTGGATAATGGCTGAACGCCTCTTAAGCCAGAAACCATGC360160.12945872550587786No Hit
GGGAAATATATTACTCGGTTCGCCGGGTATTCATATTAATTGAATTAATA359020.12904895499533617No Hit
GGAACATCAGCGAACTTGGATAATTATGATAAAACAATTCACTGAAGTTG353030.1268958625759109No Hit
GGAAAAGCTGTAGCTAATACTTGCTTTAGCACTCTTGATTCATTTCTTGA352630.12675208344940503No Hit
GGAGAAGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTA352230.12660830432289916No Hit
CGGGTATTCATATTAATTGAATTAATAAGTGGCACTTCTCATAGTGTTTC351020.12617337246521892No Hit
GAAAGATCTCTCAGGATCGCTGGAGTTGACTAATATAATTTTACCAGGTA350450.12596848720994808No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA348050.1251058124509129No Hit
AATACCACTACTTTTAAAGTTGTTTTACTTATCTATTGATGGGGAAATAT346150.12442286160001005No Hit
GTTCAAAGCAAACAGATATAGCATTGCGCGTTTGAATACTACAGCATGGA336940.1211123472122126No Hit
AGGGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGA335900.12073852148329735No Hit
CCCAATTCTAAAGAAGAGAGGTAGTGACAAGAAATAACAATACAAGGCCA331440.119135384222757No Hit
CTTGAATTCTTAATTTCCTGTTTTGTAGTAATAGTAATTCGTTTTTATGA330770.11889455418585969No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT328960.11824395363842066No Hit
GTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGGTTTCGGCCTTGA326550.11737768440122284No Hit
GATATGTTAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATG324080.11648984829504913No Hit
GTTCTTTTAATTAAAATGATTCTTTTTAAGGACTTTCTTTGCTTCGGCTT323460.11626699064896506No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGACATTTTAAGCAACGC322900.11606569987185685No Hit
GGACATTAGAATAGAGAAGAAAAGAAAATGGTTGAAAATAAAGGTATCGA322780.1160225661339051No Hit
GAGAAATTATACGTGCTTCCTGTACGGATGCATTCATAATCCTATCACCT322280.11584284222577276No Hit
GCTGAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCAT320130.11507002942080377No Hit
GTTAAAACGCTCGTAGTTGAATTTCAAAGAATCGATATTTTAAGCAACGC320060.11504486807366522No Hit
CTAGCAAATGTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGCT319210.11473933742984029No Hit
CTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAAT313170.11256827261960176No Hit
ATTTTTTTGGGGTTTCGGCCTTGAATTCTTAATTTCCTGTTTTGTAGTAA312290.11225195854128887No Hit
AACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAAACTGAAATATGTTT310510.11161214142833778No Hit
GTTGTTTTACTTATCTATTGATGGGGAAATATATTACTCGGTTCGCCGGG309520.11125628809023579No Hit
ATTCAAGCGAAGTGAGCAGGAAAGCTCTCCCTAAGAGGGTGAAAGGCCCG307510.11053379797954382No Hit
GGCTGAACGCCTCTTAAGCCAGAAACCATGCTGATTAAACAATACTATCT305840.10993352012638184No Hit
ATTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTTCAC304450.10943388766177398No Hit
GCATAAGTGGGAGTAGAGACTGAAATATGTTTTTACGACAGTGAAATACC301540.10838789451644384No Hit
ATACAAATGAACTCCTTCACATAGGTCTTACACTCGGGGTGCGTTTTCTT300860.10814347000138388No Hit
CGGGTATTCATATTAATTGAATTAATAAGTGGAACTTCTCATAGTGTTTC298920.10744614123783046No Hit
ACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTT298000.10711544924686697No Hit
ATCTACTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTA297330.10687461920996964No Hit
CGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAAACTGAAATATGTTTTT297020.1067631903869276No Hit
GAACAAGGACCAGCTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACC296950.10673802903978907No Hit
CTTGACATTTTTTCTATAAGGATAACTACGGAAAAGCTGTAGCTAATACT295010.10604070027623565No Hit
AGGGAAACAGGTTGATATTCCTGTGCCGATAGTATTTTGAGTGTAATAGA293320.10543323346674839No Hit
GAAAAGTGTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCC288680.1037653955992804No Hit
TAAACATTTGGTACTTCGCTACCTTCTATACGCACAATTAATGTAATATG285910.10276972514822728No Hit
GAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGT284500.10226290372729414No Hit
AGTATTTTGAGTGTAATAGAGATGGTAACATATATACAAATGAACTCCTT283100.1017596767845236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)