FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n02_KHC-001_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n02_KHC-001_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28476889
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAG1511190.5306724340569646No Hit
CTCAAAAGGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCA840820.2952639946027812No Hit
GGAAAATCCCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAG744080.26129258712213965No Hit
TTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGC699840.24575718225400253No Hit
GTTTTCTTTGCACTTTACCTCTATAGCAAACCTTGGAATCAATTTACTTG607850.21345379405734946No Hit
CTTTACTCTAGTCTGGCTTTGTGAAACGACTTAAGAGGTGTAGCATAAGT542030.1903403142105867No Hit
CTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCTGA470520.16522872284258297No Hit
GCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTCCAATTAATGCC441110.15490104976003524No Hit
ATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTT415640.14595695477831164No Hit
GTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAATGTTT413930.14535646783607578No Hit
CTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAAATTATTTTTTTGGGG409180.14368844855208726No Hit
CTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATTCAGGCAA406850.14287024119804662No Hit
GTCATGTTGAGTAATTTTCCAATTAATGCCTTAGCATTCAAGCGAAGTGA397520.13959390016233866No Hit
GTCCTTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAAACAGATATAGC391980.1376484629342763No Hit
GTGCAAGTGTGTATGATATGTTAAACATTTGGTACTTCGTTACCTTCTAT388710.13650016334298315No Hit
AGCAAACCTTGGAATCAATTTACTTGGAGAAGAGGTTCGCTGAATCAATT373190.13105012980877231No Hit
GTTGGTTCAGACAATTGGTACAGCAATTGGTTGACAAACCAGTGTTGCGA346520.12168464048162003No Hit
CAAGAGTTAGCCGCTCCTAAGGGATAGCTGAAAAGTGTTTATTTGGGGAA346010.12150554788481283No Hit
CTTGTTAAGCCTTTTAAGAAAAAAGTTATTAACTTAAGGAATTATAACAA339130.11908955363768844No Hit
TTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATAGCT327750.11509333059520652No Hit
GTGCAAGTGTGTATGAAATGTTAAACATTTGGTACTTCGCTACCTTCTAT325350.11425054190434918No Hit
GGTTTCTTGTCAACTGTGATTGGACAAGAGTTAGCCGCTCCTAAGGGATA319370.11215059341629628No Hit
AAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATATGTT303800.10668300178435924No Hit
ATTAAATAGTACCTGAAATCGTTAAGATGGAACGGATTAAGAGAGAAAAA297470.10446014661222298No Hit
CCTCGGTTTGTGCAAGCTTACCTTGGGAATTGTCATGTTGAGTAATTTTC295560.10378942727908234No Hit
GTGAAAGTATATGCATATTTTATATGTAGAAACTGCGAACGGCTCATTAA294990.10358926496500372No Hit
GTTTATTTGGGGAATGTTTTGTAAGAAATATGCATTTCATCCCCGTCTCG287160.10083966686108164No Hit
TGTGAAACGACTTAAGAGGTGTAGCATAAGTGGGAGTAGAGACTGAAATA286200.10050255138473868No Hit
CATAATCCTATCACCTTTTTCGTGAGCTGCTGTTCGTGAGCTGTGAGCTG286080.10046041195019584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)