FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MTH-037_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MTH-037_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28638950
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC5877152.052152750013531No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT4106371.4338409753150867No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC3878011.3541034151042548No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT3111381.086415528502267No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA2938861.026175889828363No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA2695430.9411762651912866No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG2579940.9008500660813333No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC2317210.8091113675606123No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT2223650.7764425720915047No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA2201600.7687432674731441No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA2069650.7226696509473985No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA1948800.6804718748417802No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1863670.6507466230430934No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC1806340.6307284310353557No Hit
CCCCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGG1672720.584071692572528No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT1661230.5800596739754774No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT1641320.5731076034561323No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG1600860.5589799905373626No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT1505760.525773465856814No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA1438370.502242575234078No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT1359960.47486377817622505No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT1300100.4539621738925484No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT1299840.4538713884412662No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA1298550.4534209529329812No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT1293240.45156683467794734No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA1279480.4467621892562402No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA1235770.4314997581964423No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT1193820.41685187480686264No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA1129470.394382475614504No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT1122910.3920918888436901No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC1122410.39191730143737813No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG1119980.39106880664270166No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT1090370.380729740440903No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG1048620.3661516920138483No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT1031530.360184294466103No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC1029730.35955577980337966No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA1020370.3562875035572184No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC1000360.34930051555661085No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG969930.3386751260084605No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG961480.3357245988417871No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC959700.33510306767531634No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC950760.33198144485045716No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA928080.3240621601001433No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT919090.320923078534653No Hit
AAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTA892240.3115477348156968No Hit
GGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTA868260.3031745228089717No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC867920.3030558033726795No Hit
CTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGGACT833480.2910302228259067No Hit
GTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTTTCCCGCT819750.28623605264857827No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC798230.27872181068090834No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT750780.2621534658218964No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATGTATGTTACCAT744160.2598419285623251No Hit
GAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTTTGATTTTCCTC732060.25561691332957387No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG722780.25237657106842254No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC685110.23922315587687398No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT675270.2357872757206532No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA657440.22956148881156604No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA641730.22407595250524195No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC631100.22036422424704816No Hit
CTCCCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTT615550.21493455591074392No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC578620.20203953008053718No Hit
GCCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAG570390.1991658213726411No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG565750.19754565024206547No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT565150.19733614535449098No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA561730.1961419674953167No Hit
CTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAAATAGTCCA560950.19586961114146992No Hit
CATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAA546640.19087291957281954No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC544960.1902863058876111No Hit
GCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGA521490.18209117303532427No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA518200.1809423879017911No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA490800.17137499803589168No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT478480.1670731643443632No Hit
CCCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAG473020.16516666986743578No Hit
ATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAA464260.16210789850884896No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA463320.16177967418498235No Hit
GCCACTTATTAATTCAATTAATATGAATACCCGGCGAACCGAGTAATATA460220.16069723226584773No Hit
GTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATT450260.15721945113211203No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATGTA446370.15586116111100443No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA440620.15385340593841604No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA425550.14859134151217138No Hit
GTGGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAA420360.14677912423465245No Hit
GTCAGAAACTCGATTGATACACACTAAAAATGGACGGATCCATTACGTGT409570.14301152800643877No Hit
CCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCG401490.14019019552043632No Hit
GTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAA396110.13831163502851884No Hit
ACGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGA393750.13748758247072607No Hit
GCCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAA390000.1361781769233858No Hit
ACCATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGT382460.13354539883620034No Hit
GTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATCTCG379900.13265151131588274No Hit
CTCACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGT379140.13238613845828845No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC375670.13117450185848295No Hit
CCCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGG374270.13068565712080923No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT370170.12925404038905056No Hit
GGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATC370170.12925404038905056No Hit
CTCGATTGATACACACTAAAAATGGACGGATCCATTACGTGTCGCTTCTT366770.12806684602612875No Hit
CATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCG366310.12790622561232168No Hit
CTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGTATGCGCCGACAG363250.12683775068569203No Hit
CCCCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGTGTGGGG362260.12649206762119422No Hit
CCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAA360620.12591942092849076No Hit
CCCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGG360520.12588450344722832No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG359520.12553532863460426No Hit
CCCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGGA356040.12432020028667252No Hit
GCACGTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAGA350770.1224800490241437No Hit
ACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT346540.12100303956674388No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC346290.12091574586358789No Hit
GTCACTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG342560.11961332381250012No Hit
GCCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGC330140.11527657263970921No Hit
CGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAA329090.11490993908645393No Hit
CCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACA323360.11290916741011803No Hit
CCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCT319390.1115229434040005No Hit
GCCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGT310880.10855146574856968No Hit
ATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTC310130.10828958463910164No Hit
CCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAA308850.10784264087894285No Hit
CGCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTT303820.1060862915714438No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC301820.10538794194619565No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG297890.10401568493258308No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT292160.10201491325624717No Hit
CTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAAA289060.10093247133711257No Hit
TCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCT288700.10080676840456791No Hit
GGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAAT286750.10012587751995097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)