FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MTH-031_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MTH-031_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22621981
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT2220300.9814790313898681No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA2182620.9648226651768472No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC2127930.9406470635794452No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA1830140.8090096088401807No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT1559030.6891659930224501No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC1526330.6747110255286661No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC1461540.6460707397818077No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG1370270.6057250247005336No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA1276910.5644554294338767No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC1248950.552095769154788No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT1021490.451547545725549No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT1017280.4496865239167162No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT1016370.44928426029532964No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT975550.43123986356455696No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC940320.41566651479373096No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT927740.4101055517640122No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC908670.4016756976323161No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA874820.3867123750126039No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT823350.3639601677678007No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA810210.3581516578941517No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC791000.3496599170514731No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC788160.3484045009143983No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA786390.34762207606840445No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC773380.3418710324263821No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG760070.33598737440368287No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA755160.3338169190399373No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC733540.3242598426724874No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG721390.3188889602550723No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT699430.30918158758952186No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT681300.30116725851728016No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA669930.296141173489625No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA646020.28557180734967463No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT625930.276691064323677No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA622950.27537376147561965No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG615530.2720937657935439No Hit
GTCACTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG610440.2698437418013922No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA607470.2685308594326907No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA577310.2551986936953046No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTC574590.25399632331050054No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT568100.25112743220852324No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC563490.24908959122545457No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT562460.24863428185179717No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA558300.2467953624397439No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT536350.23709241025354938No Hit
GTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATCTCG534280.23617737102687866No Hit
GGATTATGAATGCATCCGTAGAGGAAGCACGTATAATTTCTCTTTTACAT534020.23606243856362533No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATGTA521210.230399804508721No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA517650.2288261138580215No Hit
GTCAGAAACTCGATTGATACACACTAAAAATGGACGGATCCATTACGTGT510770.22578482406116424No Hit
CTCGTTATACATATCAGTGTAGCACGCGTGCAGCCTAGTTCATCTAAGGA500090.22106375210906595No Hit
GTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAAT460950.2037619959100841No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG452600.2000708956479099No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC434370.19201236178210918No Hit
GTTTAATCAGCATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAAC431420.1907083203721195No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT428290.18932471033372364No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG423600.18725150551580783No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA421450.18630110245428994No Hit
CTCGATTGATACACACTAAAAATGGACGGATCCATTACGTGTCGCTTCTT420260.18577506541093816No Hit
GCCACTTATTAATTCAATTAATATGAATACCCGGCGAACCGAGTAATATA418540.18501474296172382No Hit
GAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAATAC417110.1843826144138305No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT416190.18397593031308795No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA414830.18337474512068594No Hit
CCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCT412480.18233593247205007No Hit
GTTCAATTTTGTTATTCCATGCTGTAGTATTCAAACGCGCAATGCTATAT406660.17976321348691787No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT401020.1772700631301918No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC400010.1768235947152462No Hit
CTCGATTGATACACACTAAAAATGGACGGATCCATTACGTGTCACTTCTT398430.17612515897701442No Hit
GGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATC393570.17397680601004836No Hit
CTCACGAACAGCAGCTCACGAAAAAGGTGATAGGATTATGAATGCATCCG393190.17380882779452428No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG390620.17267276460005868No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC387990.17151017852945769No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT387490.17128915456166283No Hit
CCAAGGTTTGCTATAGAGGTAAAGTGCAAAGAAAACGCACCCCGAGTGTA378070.16712506300840763No Hit
CATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCT375160.1658387035158415No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA375080.16580333968099434No Hit
GTGGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAA368720.16299191481064368No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT366510.1620149888729904No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG365610.16161714573095964No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA364770.16124582546506425No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA351690.15546383846755066No Hit
GCACGTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAGA341180.15081791466450264No Hit
CTCACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGT339940.15026977522437138No Hit
CGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTATAA339800.15020788851338882No Hit
CTCTGACATCTGAATACAAATGCCCCCAAGCTACTCCTATTAATCGTAAC339510.1500796946120678No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT338110.14946082750224218No Hit
GCACCATTTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT334730.1479667054799489No Hit
CCCACTTGGAGTTATATTATTTGACAAACGGGCAAAATACAAATAAAGAT332760.14709587104683716No Hit
CTCCCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTT332170.1468350627648392No Hit
CATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCG331960.14674223269836537No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA331300.14645048106087616No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT329780.14577856819877977No Hit
GTTCCTCTAAGAAGCTTTACAATCAAGCAAATACATTCTTACGCGAAAAA327340.14469997123594083No Hit
CTGACATCTGAATACAAATGCCCCCAAGCTACTCCTATTAATCGTAACTA317900.14052703872397382No Hit
AGCACGTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAG316760.14002310407740154No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC316360.13984628490316564No Hit
GGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAAT309750.13692434804891757No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG309180.13667238072563143No Hit
CATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAA304140.13444445913025918No Hit
GACCTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATACAAA301370.13321998634867566No Hit
CTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGGCTTA296780.1311909863243188No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC295990.13084176845520293No Hit
CGTCATTTTGCAAATATAAAGGATCGATAGGCCACGCTTTCACGTTTTCT291720.1289542237702348No Hit
GCCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGC291280.12875972267857533No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA290480.12840608433010353No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC289240.12785794488997226No Hit
CGCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTT287620.1271418272343169No Hit
GCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCA287000.1268677575142513No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC286850.12680145032391282No Hit
CCATTATCCAACAGACTTAGCTTCGCAACACTGGTTTGTCAACCAATTGC283050.12512166816867187No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT279040.12334905594695708No Hit
CCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCG275200.12165159187429253No Hit
GTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAGAACGA270200.11944135219634389No Hit
ATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAA265360.11730184018808962No Hit
CTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAAATAGTCCA263970.11668739355761991No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA263710.11657246109436659No Hit
GTAAAATTATATTAGTCAACTCCAGCGATCCTGAGAGATCTTTCGAAAAT259400.11466723449197486No Hit
CCAGAACCCAAAGACTTTGATTTCTCATAAGGCACTGAAGGAAGCAATCT254390.11245257433467033No Hit
CAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCTTTCGAGACGGG254070.11231111899528162No Hit
ATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCTTTC253870.11222270940816367No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA252800.11174971811708266No Hit
GTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAATACTATCGG251000.11095403183302116No Hit
GCCAGTTCTACAATAATTAACATATTACATTAATTGTGCGTATAGAAGGT251000.11095403183302116No Hit
CACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCTT248160.10969861569594636No Hit
GTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAA247800.10953947843913404No Hit
CCCCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGG247090.10922562440486534No Hit
CGAGATAGTATTGTTTAATCAGCATGGTTTCTGGCTTAAGAGGCGTTCAG246920.10915047625581509No Hit
CTCTAAGAAGCTTTACAATCAAGCAAATACATTCTTACGCGAAAAAGAAA246920.10915047625581509No Hit
CCTGTTGTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAA246740.10907090762740893No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC244440.10805419737555257No Hit
CACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAAAT243950.10783759388711359No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC241670.10682972459396903No Hit
CTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATGTAC241400.1067103716513598No Hit
GCTATATCTGTTTGCTTTGAACACTCTAATTTACTCAAAGTAACAAGGAC239340.10579975290404496No Hit
CGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGGCT237880.10515436291808397No Hit
CCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAA237200.10485377032188295No Hit
CACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTCAA236500.10454433676697014No Hit
CTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACAATCAAG236340.10447360909727578No Hit
CAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGTAA233530.10323145439826864No Hit
CTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCTT231730.10243576811420715No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGGAAGATGAATT229400.10140579642428309No Hit
CTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGGACT229400.10140579642428309No Hit
ATTGTTTAATCAGCATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCC229150.10129528444038566No Hit
CCTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAA227670.10064105349571287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)