FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MTH-029_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MTH-029_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36301056
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC2819460.7766881492373114No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC2641810.7277501789479622No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC2528690.6965885510327854No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC2360520.6502620750206275No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG2250020.6198221891947165No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT2109300.5810574766750587No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG2053180.5655978713126142No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC1883590.5188802221070373No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC1699290.4681103491865361No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT1634440.4502458551068046No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG1545360.4257066240717625No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC1455270.4008891642160493No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG1260200.3471524354553212No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCC1221890.3365990234554058No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC1194380.3290207315181134No Hit
CCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG1143860.31510378100295483No Hit
CCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAG1134330.31247851302177No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT1131840.31179258256288744No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT1131240.3116272981149639No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG1109350.30559717050655494No Hit
CTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTT1056800.29112100760925524No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG1055280.290702287007849No Hit
CTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCAT1009190.27800568666652564No Hit
CCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAAT970830.2674385009626166No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC946230.26066183859775316No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA932470.25687131525870766No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG932180.2567914277755446No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA919120.2531937362924098No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC911260.25102851002461196No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA843150.23226597044449615No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC833120.229502965423375No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT763570.2103437431682428No Hit
GCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATC761760.20984513508367358No Hit
CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTC760350.2094567166310534No Hit
GTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGG743520.20482048786679927No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA739590.2037378747329003No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC729860.20105751193574095No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG725520.19986195442909432No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC715420.19707966622238207No Hit
ACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGG708880.19527806574001594No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG707960.19502462958653324No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG681050.18761162209716434No Hit
CCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCC680470.1874518471308383No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC670620.1847384274440942No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC662480.18249606843393204No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC658830.18149058804239745No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA655360.1805346929852399No Hit
GGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATC655090.1804603149836743No Hit
CGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCG636460.17532823287564966No Hit
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC632600.17426490292734184No Hit
GTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGG625260.1722429231810777No Hit
CCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCG622700.1715377095366041No Hit
TCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC619960.17078291055775346No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAG610970.16830639857969973No Hit
CCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGG598250.16480236828372155No Hit
GGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGT596100.16421009901199568No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG594040.16364262240745833No Hit
CGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAG592970.16334786514199479No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCG592480.16321288284285726No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC590340.1626233683119301No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC586880.16167022799557124No Hit
CCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACC581520.16019368692745467No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA537570.1480866011170584No Hit
GCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATT524880.14459083504347642No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG520170.14329335212727698No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT514600.14175896150238715No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG512660.14122454178743452No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT512520.14118597541625236No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC510820.1407176694804691No Hit
GGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGG506050.1394036581194773No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA500710.13793262653295815No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG498610.13735413096522592No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC483710.1332495671751257No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA482980.13304847109681878No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCAT480970.13249476819627506No Hit
TCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCT475520.13099343446096995No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG468560.12907613486505737No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG467010.128649150041255No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA466570.1285279414461111No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG465370.12819737255026412No Hit
CCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTC464640.1279962764719572No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA463790.12776212350406557No Hit
CTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCC458750.12637373414150818No Hit
GTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCC457430.12601010835607646No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT452210.12457213365914203No Hit
TCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATC450840.1241947341697167No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCC448720.12361072912038702No Hit
CCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCG448550.1235638985268087No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCC445330.12267687198961927No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA441340.121577730410928No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG438650.12083670513607096No Hit
CTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGG436360.12020586949316296No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC430050.1184676280491675No Hit
GCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC427410.1177403764783041No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG425850.11731063691370301No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC422440.11637127030133779No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC421360.11607375829507549No Hit
CCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCAT418870.11538782783619297No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC417770.11508480634833322No Hit
CGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAG414380.11415094921756545No Hit
ATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCC410940.11320331838280406No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAG406010.11184523116903265No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC405370.11166892775791426No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG402410.11085352448149167No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT401960.11072956114554905No Hit
CACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGG400940.1104485775840791No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC400050.11020340565299257No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT395410.1089252059223842No Hit
CGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGG395350.10890867747759184No Hit
GTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGC389610.1073274562591237No Hit
GCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTAT387110.10663877105944247No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA383490.10564155489030402No Hit
CACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCG379700.10459750812758725No Hit
TGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCA374610.10319534506103624No Hit
CCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATT366970.10109072309081035No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG365690.10073811626857357No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG365250.10061690767342965No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTA364080.10029460299997885No Hit
GCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGC363980.1002670555919916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)