FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MTH-021_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MTH-021_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27802123
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA4345801.5631180395828046No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC3396821.2217843939471817No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA2972041.0689975006584929No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC2704550.9727854236167505No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC2503350.9004168494614603No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC2451430.8817420166078683No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT2242590.8066254508693456No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT2176140.7827243984209408No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG2162700.7778902352169292No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT2146140.7719338555548438No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT2054200.7388644385178786No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC1667940.5999326022692584No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA1660650.5973105003527968No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC1602410.5763624598020806No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG1546000.5560726423661962No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA1492870.5369625909503386No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT1461050.5255174218170318No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC1398360.5029687840745112No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT1391020.5003286979199394No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT1318240.47415084092678816No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA1295150.4658457197675156No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC1284280.4619359464023665No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA1226220.44105264910884684No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC1222210.4396103132124119No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA1203230.4327834964257945No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA1184870.4261796841917432No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA1100510.3958366776522786No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT1074590.3865136486159708No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA1022930.36793233380055185No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT1016760.3657130788177579No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA981490.35302699725484993No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA971110.34929346942318035No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA955650.3437327429995184No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT909240.3270397731856664No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA888220.3194791994841545No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA875270.3148212818136226No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT863870.31072087552450584No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG854770.3074477441884564No Hit
CGCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTT811360.2918338286612141No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA795920.2862802959327962No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT795740.2862155526755996No Hit
CACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAAAT782280.28137419577634415No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG762890.27439990823722343No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA726600.2613469482168682No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC724950.26075346835923285No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC715900.25749832126129363No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT707140.2543474827443933No Hit
CGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGA703460.25302384281948537No Hit
CATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAA693860.24957086910233436No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA693490.24943778574031916No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA693070.24928671814019385No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG691330.2486608666539602No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC685250.24647398329976453No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC671830.24164701379099715No Hit
CAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGTAA661430.23790629226408355No Hit
TCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCC655850.23589925129098954No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT651170.2342159266038784No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA638000.22947887828566182No Hit
CATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCG623670.22432459564328955No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC612850.22043280651625058No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT599010.21545476940735783No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT586840.21107740585134452No Hit
CCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCT586470.21094432248932932No Hit
ATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCTTTC570190.20508865456066072No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC565010.20322548749244795No Hit
ACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGTAT560440.2015817281291792No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC546060.19640946124869674No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA527630.18978047108129117No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGAAAA516210.18567287109693026No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG505180.18170554816982862No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT496520.1785906781291486No Hit
ATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAA489950.1762275492414734No Hit
TACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA485650.1746809047639995No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAACGCAATTT476450.17137180495172977No Hit
CCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTG472240.16985753210285415No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC467290.16807709252994815No Hit
ACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT463090.16656641652869458No Hit
CACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCTT462060.16619594122362527No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG454330.1634155780117943No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC453740.16320336400209437No Hit
CTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCTT440440.1584195566647914No Hit
GTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAGTGAA432560.15558524073862992No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG431750.1552938960812453No Hit
CCCACTTGGAGTTATATTATTTGACAAACGGGCAAAATACAAATAAAGAT426500.15340555107967835No Hit
CCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCG425290.15297033251741243No Hit
CCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAA425170.15292717034594805No Hit
CCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAA420110.1511071654491997No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT417140.15003890170545608No Hit
AATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAA412220.14826925267541619No Hit
TCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAA410010.14747434935094705No Hit
CTCCAATTGAGTTTTGGGAAGGTTTTAAATTCCCATCATTCCAATTACAA393880.1416726341366089No Hit
CGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAA392120.14103958895513122No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT392060.14101800786939903No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA391370.1407698253834788No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT385660.13871602539129838No Hit
CCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTC379520.1365075609513705No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA379040.13633491226551295No Hit
GGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTA378730.13622340998922997No Hit
CGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAA363490.1307418142132527No Hit
CTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGTATGCGCCGACAG357400.128551334011435No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATGTA341180.1227172471684986No Hit
GTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGAT336800.12114182791004846No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA336390.12099435715754513No Hit
ATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCTA335410.12064186609058597No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG333490.11995127134715575No Hit
CCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAG330200.11876790847950712No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC326750.11752699604990596No Hit
GCACCATTTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT316500.11384022723732286No Hit
TTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGCA303350.10911037261435036No Hit
CAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAA300030.10791621920383562No Hit
CCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGTC297840.10712850957461054No Hit
GCCACTTATTAATTCAATTAATATGAATACCCGGCGAACCGAGTAATATA297730.1070889442507682No Hit
GCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTTT297170.10688752078393438No Hit
CCCCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGG291960.10501356317285553No Hit
ATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGT290160.10436613060088973No Hit
CTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAAT283840.1020929229037653No Hit
CGGCACAGGAATATCAACCTGTTTCCCTTTCGAGACGGGGATGAAATGCA283030.10180157824638068No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC281780.10135197229362665No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC278550.1001901905117102No Hit
AGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAG278180.10005710714969501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)