FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MTH-019_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MTH-019_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43856866
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCAT2092430.4771043147497133No Hit
CTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAAT1663110.3792131430458346No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA1532880.34951881878654983No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT1319810.3009357759398494No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC1133790.25852052447158447No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCT1093930.24943186774905438No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC1062540.2422744935764448No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCC1021520.23292134007021842No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC1015160.23147116804926282No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC1014290.2312727954614906No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC1010510.2304109007697905No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATAGGTATCAAATTAAAGTAATAA937050.21366095789881567No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC933620.21287886827116193No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC911450.20782378750000058No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC877050.1999800897765928No Hit
CGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAA822480.18753734021943108No Hit
GCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAAA814730.18577022808697732No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT780530.17797213325731026No Hit
CCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATC754930.17213496285849517No Hit
CTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACC746120.17012615538921544No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA739350.1685824974360913No Hit
CGCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTT687300.15671434434006296No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGACAAATGCTTTCGC649640.14812731944868107No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC642350.146465093971831No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCAATC625060.14252272380794379No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTG620360.1414510558050363No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT617710.14084681746297148No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCCTAGCT613750.13994388016690476No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG608310.13870348145715658No Hit
CTTAAACTTCCTTGTGTTAGACACACAATGTTCCTCTAAGAAGCTTTTCA606310.13824745251974913No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC605850.13814256586414542No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGTAAGTAATTTA605070.13796471457855652No Hit
CCCACTTGGAGTTATTTTATTTAATATGAGTGAAATACAAATTGAGTTAT604300.13778914343765467No Hit
GTACGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAA574600.13101711371715433No Hit
CCATGCTATAGTATTCAAACACAGTAAATGCTTTAACTGTTTGCTTTGAA552010.12586626686913743No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT546850.12468971221062626No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATATA532040.12131281792912427No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACAATGTTCCTCTAAGAAGCT519810.11852420097687782No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC513900.11717663546683887No Hit
CTCTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTA510140.11631930106451292No Hit
CTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAAAAACG508510.11594763748052585No Hit
GTCAGTATCAACTCGATCCTCCATCAGACTTTCGTCTGACTTCAATCTCA501300.11430365316117207No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA500820.11419420621619429No Hit
GTTACTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTATAAGG494570.11276911578679608No Hit
CCCCGTTCAAGAAATTTTTTGAATTGAGTTCAACGAACCTCTTCTCCAAG492010.11218539874691456No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT468020.10671533164271245No Hit
GTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACCGGTAT462490.10545441163078093No Hit
CTCCACTTCAGTCTTCAAAGTTCTCATTTGAATAGTTGCTACTTCCACCA461450.10521727658332905No Hit
CCAGAACCCAAAGACTTTGATTTCTCATAAGGTACTGAAGGAAGCAATCT451340.1029120503047345No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT444790.10141855553472516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)