FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-092_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-092_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23168990
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC3606211.5564813140322475No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT2624811.1328978949880855No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC2575711.11170577569415No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA2209840.9537921161000112No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG2187560.9441758143104209No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA2099980.906375288694069No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC2075060.8956195328324627No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT1905010.8222240158073355No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT1656780.7150851202404593No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA1403960.6059651283892824No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1253630.5410809879930027No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA1216680.5251329470986866No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC1215920.5248049224415912No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT1194100.5153871618918218No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA1162990.5019597315204504No Hit
CCCCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGG1069360.4615479569890617No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA991200.4278132106751309No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC953210.411416293934263No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT935700.4038587784793381No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA926060.39969804467091574No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG921970.3979327540820726No Hit
CTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGGACT828360.35752961177850223No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA784340.33853007835041576No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC770170.3324141449411476No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT730680.315369811113907No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATGTATGTTACCAT729460.3148432452169905No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT707470.30535211073076557No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC693620.29937429296659024No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT651090.28101786051096744No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT647400.27942521447849045No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG638640.2756442986940734No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT625480.2699642927896296No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA604100.26073644125186296No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG597030.25768494871809255No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT591280.2552031832203303No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA591100.2551254931699656No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG575570.24842256826905273No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT572230.24698098622339604No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC558180.24091684618103765No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG549450.2371488787383481No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA505510.21818387422153493No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC502820.21702283957997304No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT499240.21547767080049668No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT498590.21519712339640182No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA496670.2143684295258447No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA494070.21324623990946517No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG493760.21311244037828148No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC485220.20942647909986584No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT483790.2088092748108571No Hit
CTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAAATAGTCCA480630.20744538281556513No Hit
GCACCATGTAGGAACAACTCAAACATCAAAGAAATAAATCAATGCAATTT459720.19842038863152864No Hit
GTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATCTCG459500.19832543412552725No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA450320.1943632415569259No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC402960.17392212608318272No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT401560.1733178701359015No Hit
CTCACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGT392540.1694247353898465No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA391660.16904491736584115No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC387560.1672753106630889No Hit
AAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTA378320.16328722141103258No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA372470.16076229477417875No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT362780.15657998039621063No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA360220.15547505523546776No Hit
ACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT339650.1465968089243424No Hit
GTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAA336440.14521133635950467No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT335650.14487036336068168No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT335330.14473224771558882No Hit
CTCTGACATCTGAATACAAATGCCCCCAAGCTACTCCTATTAATCGTAAC335260.14470203491822473No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA327260.14124914379090328No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT315180.13603527818864786No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATGTA314070.135556189544732No Hit
GACCTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATACAAA313530.13532311939363778No Hit
CCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAA304730.13152493915358415No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA304180.13128755288858082No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC300840.1298459708429241No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC290990.12559459864240952No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG286420.12362213458592713No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACATCAAAGAAATAAATCA284880.12295745304391775No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC284560.1228193373988249No Hit
CTGACATCTGAATACAAATGCCCCCAAGCTACTCCTATTAATCGTAACTA282790.12205538523690503No Hit
GTTCAATTTTGTTATTCCATGCTGTAGTATTCAAACGCGCAATGCTATAT281380.1214468131757146No Hit
CAAAAAAATAATTTGCCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAA277080.11959088419477933No Hit
CTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAAA275240.11879671923549538No Hit
CAGCAAATAAGATAATTCCCCTTTTGAGAAGAGTTATAAAACCAGTTGTC268670.11596103239718261No Hit
GGATTATGAATGCATCCGTAGAGGAAGCACGTATAATTTCTCTTTTACAT268000.11567185276526945No Hit
ATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCTA265830.1147352560469835No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG265160.11444607641507032No Hit
GCACGTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAGA259810.11213695547367408No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA258770.11168807962712228No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG255250.11016880753110082No Hit
CCCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGGA253410.10937464257181691No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG252490.10897756009217494No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA246860.10654758796132245No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC246590.10643105288577534No Hit
TACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA242920.10484703908111662No Hit
GTCACTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG238660.10300837455581792No Hit
CACGTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATC233660.10085031760124201No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC233110.10061293133623865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)