FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-086_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-086_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22649403
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAATTGAGTTTTGGGAAGGTTTTAAATTCCCATCATTCCAATTACAA5663602.5005515597916643No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT2400491.0598469195854743No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA2013210.8888578652602896No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC1764660.7791198734907053No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT1467330.6478448902163116No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA1422350.6279856471272113No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC1372910.6061572572133579No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT1273570.5622973815248022No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG1234270.5449459308044455No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA1223210.540062799889251No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA1167450.5154440494524293No Hit
CAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGTAA1127210.49767757675555513No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC1115510.4925118776861359No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC1090200.48133719021203336No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC1083120.47821128000592333No Hit
CATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCG1071340.473010259917226No Hit
CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCC1050700.4638974369434815No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT1033380.45625043626977724No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC1013740.44757912603700856No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC991590.43779961882439017No Hit
CCTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGCTCC945840.41760041092473826No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT874330.38602783481754466No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT862460.38078707858215954No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT806570.35611093148901096No Hit
CATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAA799200.3528569825880179No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG791040.3492542386216537No Hit
CACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAAAT787360.3476294717348621No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGCT780690.3446845817525522No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAAAAAAAAGAA766190.3382826470084002No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC700290.3091869573780819No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA696200.30738117026749007No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACAACGCC673360.29729701926359825No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA657650.29036085410286533No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC651030.28743803975760424No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA648890.28649320249191557No Hit
CCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTC632620.2793097901962361No Hit
CCCTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACA611920.27017047645803294No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA610980.2697554544815155No Hit
CCCTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACG604650.26696067883113744No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT604120.2667266770784201No Hit
CTCCACTTCAGTCTTCAAAGTTCTCATTTGAATAGTTGCTACTTCCACCA598720.2643425082771497No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT587670.25946379248936496No Hit
CCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAG555860.24541927219891846No Hit
ATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAA551250.2433838984630191No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA543750.24007255290569912No Hit
GTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAGTGAA540880.238805411339098No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC538930.23794446149419476No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA529530.2337942417290204No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAA525050.23181626464944793No Hit
CCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCG512160.2261251654182673No Hit
ATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGT510480.22538342401342762No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT510190.22525538531854458No Hit
CCTCACGGTACTTGTTTGCTATCGGTCTCCTACACATATTTAGCTTTATG505550.22320676620041596No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT504810.22288004677209375No Hit
GCTACTTCCACCAAGATCTGCACTAGGGATATGTTCCACGAAAACTCACG489860.21627943129450253No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC478110.21109165658803455No Hit
CTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACAAC473780.2091799064196085No Hit
CTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGAC468900.20702532424364561No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA468030.2066412081589965No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC465860.20568312551107862No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA433410.19135603706640744No Hit
CTTCCGTCAATTCTTTTAACTTTCTCGCTTGCGCGAATACTCGCCCCAGA425330.18778861411932138No Hit
CCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAA414090.18282601091075115No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT411350.18161626600047692No Hit
CGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGA410010.1810246389275691No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT404520.17860073397961085No Hit
CGCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTT400040.1766227569000384No Hit
CCCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTG383920.16950557151550527No Hit
CATCATTCCAATTACAAAGCCAGATTGGCCTTGTATTGTTATTTCTTGTC379740.1676600482582256No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG377180.16652977564132707No Hit
GTAAAATTATATTAGTCAACTCCAGCGATCCTGAGAGATCTTTCGAAAAT376880.16639732181903424No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA376130.16606618726330227No Hit
CCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCT375390.16573946783498No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC374470.1653332761132821No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA367660.16232657434723555No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC367190.16211906335897683No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC361430.1595759499709551No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA356870.15756265187210455No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT356190.15726242320824085No Hit
CACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGCTCCAATC341450.15075452540625464No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT340670.15041014546829334No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGGAAGATAAATT338560.149478553584834No Hit
CCCCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGTGTGGGG333070.14705464863687576No Hit
CACGATTTTCTACTCATAAAAGGATACTAAAAAGAAGAAGTTCTTTTCTT327450.14457334703259064No Hit
GTCACTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG325080.14352696183647756No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC321360.14188453444004684No Hit
AGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAGTGA321260.14184038316594924No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG320830.14165053268732955No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG320660.14157547552136363No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT315310.13921338235714203No Hit
CTCCCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTT311670.13760627597998942No Hit
CCACGATTTTCTACTCATAAAAGGATACTAAAAAGAAGAAGTTCTTTTCT310750.1372000842582915No Hit
CACGATTTTCTACTCATAAAAGGATTCTAAAAAGGGGAAACCCATTTTCT310530.1371029514552768No Hit
GCTCCCTCTCCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCAT307950.1359638485835587No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA297270.13124849250993503No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA294140.12986655763068017No Hit
CACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCTT291180.1285596799173912No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTG289530.1278311838947808No Hit
CCACGATTTTCTACTCATAAAAGGATTCTAAAAAGGGGAAACCCATTTTC287010.1267185717875213No Hit
CCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTG285780.1261755111161208No Hit
CTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGTGTGGGGACT285560.1260783783131061No Hit
ACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGTAT283360.1251070502829589No Hit
CTCCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGC281300.12419753403654835No Hit
CCCGCTTTACTTTTTTTCTCTCTTAATCCGTTCCATCTTAACGATTTCAG280250.12373394565852354No Hit
CCCTTACCTACTTATTTCTACTTAACCAGAGGCTGTTAACCTTGGAGACC279270.12330126317236707No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG278990.12317763960489378No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC278390.12291273196030819No Hit
CCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACA270760.11954398974666132No Hit
CTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGCTCCA261190.11531871281552102No Hit
CTGCTGCCTTCCTTAGATGTGGTAGCTATTTCTCAGGCTCCCTCTCCGGA261170.11530988256070147No Hit
CAATTGAGTTTTGGGAAGGTTTTAAATTCCCATCATTCCAATTACAAAGC259780.11469617985074484No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT259770.1146917647233351No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG252280.11138483429342487No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA247190.10913753444185703No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG241920.10681076229691353No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT241640.10668713872944023No Hit
CCCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGG233760.10320801833054938No Hit
CCGCCGTTTACCTGCGCTTGATTGAATTTCATCAAGTTGACATTCAAAGC226610.100051202232571No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)