FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-081_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-081_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28365736
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC1348950.47555614280553127No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC1234280.4351306096905083No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC1071900.37788548832295416No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT976650.34430624327886294No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC876080.30885149604438256No Hit
CCCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCAT799230.2817589503053966No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCT635210.2239356666084744No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA600600.21173432623077365No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC576410.20320643187259446No Hit
CTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAAT576030.20307246743042381No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC548560.19338824841350846No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG541670.1909592615541511No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCC505260.1781233527661683No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC488310.1721478335693458No Hit
GGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGT475940.16778693843868533No Hit
CCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAG470750.16595726618903878No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC461650.16274917033705735No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC458460.1616245740988353No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC425440.14998376915021702No Hit
CTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTT399860.1409658469640978No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG394560.13909739553382292No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG392560.13839231952239844No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC385920.13605146716446914No Hit
CCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCG374200.13191972173752164No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC365070.12870104974536886No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC363400.1281123112758294No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA361470.12743191292480477No Hit
CGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAA347700.12257746458614717No Hit
CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTC336070.11847744757971378No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG335370.11823067097571521No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC330980.11668302913063847No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG329150.11603788458018505No Hit
GCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAAA326670.1151635903260187No Hit
CTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCAT323400.11401079104733966No Hit
CCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGG323380.1140037402872254No Hit
CTCCAATTGTTACTCTGGGAAGGTTTTAAATTCCCACCATTCCAATTACA311840.10993545170130611No Hit
CCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCC310760.10955471065513689No Hit
GTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGG307360.10835608143571526No Hit
CCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTC303010.106822541110867No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCG301470.10627963258207013No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT299020.10541591446807513No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCCTAGCT284150.1001736743231341No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)