FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-076_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-076_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32710862
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA12698213.881955174400479No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT4935141.5087159733057478No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC4503141.3766497501655568No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG4323291.3216680135179562No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA4039391.234877271042261No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA3411551.0429410267451833No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA3127740.9561777980659757No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA2876890.879490733078205No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC2495050.7627588658470694No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC2428430.7423925422692927No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT2342140.7160129256147393No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC2318970.708929651563447No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC2279700.6969244650293838No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAATAAATAGAA2155160.658851484867626No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT1861630.5691167661677641No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA1789070.5469345320218097No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA1761170.5384052551106724No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT1733000.5297934368100724No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT1666880.5095799676572265No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1652630.5052236165466994No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG1563260.47790241663457234No Hit
CAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC1430190.43722173998349534No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC1310040.4004908216726297No Hit
CCCACTTGGAGTTATATTATTTGACAAACGGGCAAAATACAAATAAAGAT1271360.3886660033599848No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC1254250.38343532493885363No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT1241840.3796414781120718No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA1197320.3660313201162354No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT1176800.3597581745170763No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATGTATGTTACCAT1133360.3464781820790904No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA1115580.341042678728552No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA1076460.329083348521968No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT1024040.31305809061222534No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA1018700.3114256053539647No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT1000460.30584947593248996No Hit
CTACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA936670.2863483083998214No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT905290.2767551646911659No Hit
CCTACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC880100.2690543587631533No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT844630.25821086585856406No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT828540.2532920104642916No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA803850.24574405896120988No Hit
GCACCATTTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT800750.24479636152663908No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTTATGAAATAA799290.2443500266058412No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG786740.2405133805400787No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA785820.24023212839820607No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA769540.23525518832245998No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT758290.23181596376151753No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC747340.2284684518555335No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG732490.22392867543508943No Hit
GTCTCCTACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG729730.22308491900947153No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA719250.21988109026292246No Hit
TCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAA712290.21775335666788603No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT685260.20949004645612826No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG672280.2055219455849253No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG668660.20441527954842645No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA661010.20207660684698558No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA659910.20174032711213785No Hit
CCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC653010.19963093604809315No Hit
TACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA648520.19825830331221478No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC645000.1971822081607021No Hit
CTCCTACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT631980.19320187893550467No Hit
GCCCACTTGGAGTTATATTATTTGACAAACGGGCAAAATACAAATAAAGA595150.18194262199510364No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG588000.17975680371859354No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTTATGAAATAATTA579400.17712770760978416No Hit
CGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAA574520.1756358484224598No Hit
TCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC572190.17492354680228236No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC567620.17352645735841507No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC558180.170640565815722No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG551920.16872682841558867No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG546600.16710045733432521No Hit
ACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT542590.16587456484638038No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC527250.16118499108950415No Hit
CCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTGAC522100.15961059051271714No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA517920.15833272752029587No Hit
AATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAA509620.15579534406644496No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA497530.15209932407161877No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA492730.15063192159228334No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT486250.14865092824518045No Hit
GTCAGAAACTCGATTGATACACACTAAAAATGGACGGATCCATTACGTGT475270.1452942450737006No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTTATGAAATAATTAA475270.1452942450737006No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC468680.14327962375311296No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG467770.14300142869973895No Hit
CGGCACAGGAATATCAACCTGTTTCCCTTTCGAGACGGGGATGAAATGCA465360.1422646703715726No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTC463090.14157071128238688No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT450550.137737122305123No Hit
CAGGGACGTAGTCAGCACAATCTGATGAATCATGCTTACTAGGCATTCCT446820.13659682829513942No Hit
TCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCT443510.13558493200209765No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA442550.13529145150623056No Hit
TCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTAC431210.13182471314880054No Hit
GCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGA422780.12924758754446763No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAATAAATAGAAAG416420.12730327925934817No Hit
ACGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGA405330.12391296811438354No Hit
CCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAA404680.12371425736197351No Hit
CGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAA394700.12066328304035522No Hit
AGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAG394670.12065411177485937No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG394380.12056545620839952No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA382890.11705286152349026No Hit
TCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAATAAATAGAAA376790.1151880375393348No Hit
CGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAATTTACTCAAAGTA376270.11502906893740678No Hit
CCTATGTGAAGGAGTTCATTTGTATGTATGTTACCATCTCTATTACACTC370330.11321315836922916No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAAGA367910.1124733429525642No Hit
GCTATATCTGTTTGCTTTGAACACTCTAATTTACTCAAAGTAACAAGGAC362470.11081028680931734No Hit
TCTCCTACAAATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGC360570.11022943999458039No Hit
CTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTA350580.10717540858446346No Hit
TCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCC339130.10367504225354868No Hit
TCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAA339080.1036597568110556No Hit
TATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTGACTT336880.10298719734136018No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT330880.10115294424219087No Hit
TTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTC327860.10022970351560898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)