FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-071_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-071_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35375318
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA3962701.1201878100431493No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC3881871.0973385454796476No Hit
CTCCAATTGAGTTTTGGGAAGGTTTTAAATTCCCATCATTCCAATTACAA3312670.9364353982627096No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC3155560.8920230766547456No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT2987820.8446058350627407No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT2691790.7609231950932568No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC2652110.7497063347953508No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT2581150.7296471511577649No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA2262390.6395391272525098No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG2229930.6303632380067933No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT2171080.61372734515065No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC1976340.5586776633357755No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC1933400.5465392565517009No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA1857430.5250638312283158No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT1643760.46466296076829616No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC1532630.43324840217690763No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA1472140.41614890924796777No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT1422440.40209956557846355No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC1414000.3997137213013887No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT1408160.39806285274947917No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC1355960.38330680165193143No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG1353230.38253507714050794No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA1335700.3775796446550671No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC1296850.36659741122327155No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA1286650.36371404491685416No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAA1276680.3608956956938168No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA1242150.35113465269768035No Hit
CATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAA1195000.3378061506047804No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT1175390.33226273753920743No Hit
CATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCG1137890.3216621261185553No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA1066190.3013937570822685No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA1024450.28959456986365467No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG1023440.28930906006272505No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA1012810.2863041400786842No Hit
CAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGTAA1008520.28509143013216165No Hit
CACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAAAT992340.28051761965786426No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT938470.26528948799838353No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTTATGAAATAATTA900560.25457297655953226No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC895550.25315673487373314No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA861630.2435681284900393No Hit
CGCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTT858060.24255895028279323No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA835970.2363144834486011No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT831050.23492368323021154No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT819220.23157954368070982No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT803350.22709336492748983No Hit
CACGATTTTCTACTCATAAAAGGATACTAAAAAGAAGAAGTTCTTTTCTT801600.22659866972785941No Hit
CCACGATTTTCTACTCATAAAAGGATACTAAAAAGAAGAAGTTCTTTTCT790340.2234156594719516No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC769970.2176574073482534No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA755230.21349066035250905No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC748130.2114836112568656No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT743760.21024828667264558No Hit
ATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAA731720.2068447836991882No Hit
CCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCG731690.20683630321005173No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA718100.20299464163120737No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGAACGACGCC717700.20288156844272043No Hit
CCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCT713500.20169429996360738No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA707710.20005756556025872No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC701500.1983021043089987No Hit
CCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTC697440.1971544114458561No Hit
CGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGA686100.19394878655225092No Hit
CCTCACGGTACTTGTTTGCTATCGGTCTCCTACACATATTTAGCTTTATG670740.1896067761143518No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG669460.18924494191119356No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT664620.18787675633050138No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG642690.18167751877170404No Hit
CCCTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACG623280.1761906423003745No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT622620.17600407153937103No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTTATGAAATAATTAA616580.174296666393218No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC612580.17316593450834847No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTTATGAAATAA609700.17235180755124235No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA592040.16735962627954326No Hit
CCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAA591900.16732005066357283No Hit
GTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAGTGAA586690.16584727238353023No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA584180.1651377381257746No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC583500.16494551370534677No Hit
CCCTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGAACG578260.16346425493616762No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC576930.16308828658444852No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA570800.16135543997088592No Hit
TCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCC568070.16058371545946243No Hit
CCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAG552050.15605513426055986No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT547940.1548933072488564No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT547500.15476892674152073No Hit
GTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTTTCCCGCT514150.14534144965142082No Hit
CACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCTT497830.140728063561153No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG487160.13771183625826347No Hit
ACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGTAT486620.13755918745380608No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA485540.13725388984489129No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT484040.1368298653880652No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG475920.13453447966178No Hit
ATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGT474240.1340595722701348No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC469740.13278749889965655No Hit
GCTCCCTCTCCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCAT468660.13248220129074176No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA464530.13131472061961394No Hit
CTCCCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTT462120.13063345465898002No Hit
CCCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTG459840.12998893748460438No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT456560.12906173733901136No Hit
CCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTG455120.1286546738604583No Hit
GTAAAATTATATTAGTCAACTCCAGCGATCCTGAGAGATCTTTCGAAAAT454450.12846527626974266No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT453430.1281769396391009No Hit
CTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGAC453050.1280695201100383No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGGAAATGAATTC441050.12467732445542963No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA436450.12337698278782963No Hit
CTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGTATGCGCCGACAG431890.12208794843907833No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC428860.12123141903628966No Hit
CCGCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGG422810.11952118706042443No Hit
CTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGAACGAC422100.1193204821508601No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC421810.11923850408920705No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT419820.11867596497648444No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA415780.1175339257727662No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGGAAGATGAATT412060.1164823451198375No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATGTA410810.11612899140581577No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC410360.11600178406876796No Hit
CCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAA407890.115303557129861No Hit
ACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT405100.11451487164016447No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA400870.11331912267191493No Hit
CTCCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGC397220.11228732982697144No Hit
ATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCTTTC396480.11207814442827059No Hit
TACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA387850.1096385903866645No Hit
CTCCACTTCAGTCTTCAAAGTTCTCATTTGAATAGTTGCTACTTCCACCA381720.10790574377310191No Hit
CCCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGG373590.10560753121710453No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA359220.10154537692071065No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA356000.10063513775339067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)