FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-069_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-069_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24051999
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCAT2011300.8362298701243085No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCT1476620.6139281811877674No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCC1332780.5541244201781316No Hit
CTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAAT1167830.48554384190686184No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCCTAGCT1083480.4504739917875433No Hit
CGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAA977250.4063071846959581No Hit
GCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAAA941630.39149760483525714No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC930500.38687013083611055No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC858910.357105453064421No Hit
GTACGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAA730070.30353817992425497No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA676080.2810909812527433No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC642740.2672293475482017No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT611280.2541493536566337No Hit
CCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATC590050.2453226444920441No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCC563430.23425495735302498No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTG523240.21754532752142555No Hit
GCCGTTCCCTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCA521950.21700898956465114No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA515520.21433561509793841No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA489720.20360885596245035No Hit
CGCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTT481130.20003742724253398No Hit
CTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACC466230.19384251595886062No Hit
CCCCGTTCAAGAAATTTTTTGAATTGAGTTCAACGAACCTCTTCTCCAAG450590.18733993794029344No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCAATC449820.18701979822966067No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC446610.18568518982559412No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT437640.1819557700796512No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGTAAGTAATTTA435030.18087062119036343No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAAATTGGGAGAAA425920.17708299422430543No Hit
CTCGGCATGAAAAATTCCAATGAATAAATTAAAACGAACTATGGGCCTTT412960.17169466870508351No Hit
CCCACTTGGAGTTATTTTATTTAATATGAGTGAAATACAAATTGAGTTAT397780.16538334298118007No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATATA379770.15789539987923665No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTTGCCTAGCTC378510.1573715348982012No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC375950.15630717430181168No Hit
CCACGCTTTCACGTTTTCTACTTGTTCTGAAAATAGAAATCAAACAAGCT375460.156103449031409No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC372000.15466489916285128No Hit
GTCAGTATCAACTCGATCCTCCATCAGACTTTCGTCTGACTTCAATCTCA343130.14266173884341174No Hit
CGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATCT341990.142187765765332No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC340560.14159322058844256No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC322580.1341177504622381No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG320900.13341926382085748No Hit
GCCACGCTTTCACGTTTTCTACTTGTTCTGAAAATAGAAATCAAACAAGC316570.1316189976558705No Hit
AAGAAACTCATAATACTATTGGCACAGGAATATCAACCTGTTTCCCTTTT307570.12787710493418863No Hit
CCCATCACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGA302130.12561533866686092No Hit
CTCTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTA295660.12292533356582962No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCA292590.12164893238187811No Hit
GCCCACTTGGAGTTATTTTATTTAATATGAGTGAAATACAAATTGAGTTA291590.12123316652391347No Hit
TAAGAAACTCATAATACTATTGGCACAGGAATATCAACCTGTTTCCCTTT272030.11310078634212482No Hit
CTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAAAAACG262840.10927989810742965No Hit
CACGTATTAGTGCTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTC260460.10829037536547378No Hit
GTAAAATTATATTAATCAACTCCAGCGATCCTGAGAGATCTTTCGAAAAT258540.10749210491818166No Hit
CATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCT256260.10654415876202224No Hit
GGGCCTTTCACCCTCTATGGGGAAGCATTCCTGCTCACTTTGCTCAAATT251450.10454432498521225No Hit
CCAAGGTTTGTTATAAAGGTAAAGTGCAAAGAAAACGCACCCCGAGTGTA251320.10449027542367684No Hit
CCTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATAGCAAAG250240.104041248297075No Hit
GACCTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATAGCAA244220.1015383378321278No Hit
GTTCCCTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAA241140.10025777898959667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)