FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-068_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-068_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26845743
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC3622151.3492455768499312No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC3211491.1962753275258575No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA2800371.04313372887463No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT2781621.036149381300417No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT2078180.7741190102281765No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA1832370.6825551447765852No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA1756040.6541223314251351No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT1398250.5208460797676563No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG1349490.5026830510893292No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT1344850.5009546578762971No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC1262510.47028312831572594No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1209280.45045503117570634No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA1139590.4244956081118708No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA957060.35650345010007733No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT929960.3464087397394812No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA898750.3347830603906176No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA872810.3251204483332795No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC841700.313532018838145No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA818510.3048937777583582No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC807850.3009229433508322No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT798930.2976002563981932No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA779370.29031418500877404No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC728300.27129068470930384No Hit
CGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTATAA728040.27119383508960804No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA724550.2698938151944612No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTC722610.26917116803211594No Hit
TCACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTA705620.2628424178835356No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA694460.2586853342073639No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA649660.24199739973671058No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA620980.23131414168719414No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA616210.22953732366431429No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC596010.22201285321102865No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT583730.21743857117309065No Hit
TAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAAT578940.21565430317946496No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT571780.21298721365245876No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC567840.21151956941553082No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG552770.20590601645854986No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA541210.20159993336746168No Hit
TGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAATA530120.19746892458890036No Hit
CACGTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATC521140.1941238877240239No Hit
CCAAGGTTTGCTATAGAGGTAAAGTGCAAAGAAAACGCACCCCGAGTGTA513960.19144934822627185No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA512730.19099117502540347No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG509810.18990347929651266No Hit
CCTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAA505100.18814901118587032No Hit
CATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCT489540.18235293394561664No Hit
CAAAAAAATAATTTGCCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAA488990.18214805975010637No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC487140.18145893745611735No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT465710.1734762938019633No Hit
TCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCT462150.17215019900920606No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA446030.1661455225880692No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCCCTATTACACTC444310.16550482510392803No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA442870.16496842721022845No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAAAAAAAAGAA440310.16401483095476255No Hit
CGGCACAGGAATATCAACCTGTTTCCCTTTCGAGACGGGGATGAAATGCA434780.16195491404354126No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT429410.15995459689828664No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC423300.15767863083543637No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT422790.15748865658141778No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC420030.1564605606184936No Hit
GCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCA414430.1543745688096619No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG410990.1530931738413796No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC403830.15042608431437343No Hit
GTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATCTCG400870.14932348864399098No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG390800.14557242837346687No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC386500.143970684663114No Hit
CTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAAATAGTCCA385440.14357583621358516No Hit
GTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAAT381280.14202624229845304No Hit
TCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGC365950.13631583972177636No Hit
CCCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC365310.1360774406579099No Hit
GGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAAT356660.13285532831033955No Hit
CGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAA355670.1324865547584211No Hit
CTCACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGT345220.12859394504372632No Hit
TCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTAC335190.12485778471469389No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC329110.12259299360796234No Hit
TTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTC327150.12186289647487125No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT319500.1190132826645923No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT317570.11829436048761996No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA315870.11766111297422462No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAAGA315420.11749348863244352No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAA310510.11566452081434289No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT308870.11505362321318503No Hit
TTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAA308730.11500147341796427No Hit
CCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC297760.11091516446387795No Hit
ACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT297230.11071774023911352No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA293050.10916069635323558No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC289100.1076893271309347No Hit
CCTAAACGTGACTGAAATGAACGATTTGCACGTCAGTATCAATTCGATCT289090.10768560214556178No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG287990.10727585375454127No Hit
TCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAA279170.1039904166556314No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG277610.10340931893745686No Hit
CTCACGAACAGCAGCTCACGAAAAAGGTGATAGGATTATGAATGCATCCG272180.10138665187996473No Hit
CAGCTCACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCA271340.10107375310863997No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG270050.1005932299955341No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)