FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-062_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-062_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18614269
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA3959292.1270187940230154No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT2462831.3230871435241427No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC1665840.8949263599875988No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG1537120.8257751083322156No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA1515040.8139132404286196No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC1497210.8043345672075547No Hit
CCTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGCTCC1297650.6971264893614678No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC1275520.6852377603439598No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA1248430.6706844088263686No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT1116310.5997066014249607No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA1023540.5498684906723976No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT966380.5191608652480525No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC886070.47601654408239186No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA793850.4264739055828623No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA788970.4238522608650385No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT780330.4192106603810228No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT735430.3950893800879315No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC724320.3891208405766565No Hit
CTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGCTCCA678790.3646611102482724No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA666710.3581714651271022No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA664230.35683915387706067No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA644720.3463579472285482No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTC637450.3424523412657247No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA634190.34070099663865394No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG624710.3356081294409144No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT624440.3354630794257889No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT613090.3293656065677357No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT593760.3189810999293069No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGCT586590.31512921619430767No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA544570.292555136062555No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA533180.2864361743133722No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA490510.2635128997007618No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC469670.25231718742218673No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT468750.25182294292620355No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA463830.24917980931725014No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC420780.22605239023890758No Hit
TAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAAT404330.21721508376181733No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA393940.21163334429087707No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT375780.20187738771799205No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA373350.20057193758186262No Hit
TCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTAC371100.19936318745581683No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG358060.19235780894753376No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT357980.19231483116527434No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA342710.18411144697651033No Hit
TGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAATA341900.18367629693113385No Hit
GCCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGAT338160.18166708561050665No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC327020.17568242943088444No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT323810.17395794591772581No Hit
CCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC321550.17274382356889761No Hit
TCCTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGCTC319820.17181442902753796No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG314430.16891880094781053No Hit
GTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAAT313840.1686018398036474No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC312260.16775302860402413No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT302360.16243452804942274No Hit
TCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC301880.1621766613558663No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG296840.15946906107352374No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC293420.15763176088193417No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC293400.15762101643636933No Hit
CACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTGCTCCAATC292430.15709991082647404No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA280670.15078217683434145No Hit
TCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAA278150.14942837669317016No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA275480.14799399321026252No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT267880.14391110389561898No Hit
TCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCC267220.1435565371919789No Hit
CGGCACAGGAATATCAACCTGTTTCCCTTTCGAGACGGGGATGAAATGCA262900.14123573694997102No Hit
ACGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGA255030.137007797620202No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC251450.13508454186409363No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC243600.13086734697988947No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT241260.12961024684880185No Hit
GCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGA239980.12892260233265135No Hit
CACGTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATC236530.1270691854727145No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG235610.12657494097673133No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGCATCACACGAATTAGTG235460.12649435763499497No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG235210.12636005206543433No Hit
TCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAA233380.1253769352962504No Hit
CAAAAAAATAATTTGCCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAA232930.12513518527104125No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA232500.12490417969139697No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT224350.12052581812372004No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC214750.11536848425259139No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAAGA213230.1145519063896627No Hit
TCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCT211410.11357416184326122No Hit
GCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCA207670.11156495052263402No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT206110.1107268837685756No Hit
CCAAGGTTTGCTATAGAGGTAAAGTGCAAAGAAAACGCACCCCGAGTGTA205230.11025412816372214No Hit
CGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAA203270.1092011724983667No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC202580.10883048912637934No Hit
CGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAA198410.10659027222610783No Hit
CATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCT195950.10526870542163112No Hit
GTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATCTCG193900.10416739975123385No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG193560.10398474417663138No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA192200.10325412187822149No Hit
CCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTGAC191820.10304997741248931No Hit
GGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAAT191010.10261482736711282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)