FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-054_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-054_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18128433
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC344700.19014329589325232No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC338900.18694390188054313No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC311680.1719288148071044No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA306840.1692589756654643No Hit
GGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGT288150.15894920426933756No Hit
CTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAAT247460.1365038004112104No Hit
CCCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCAT228620.1261112860664791No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT205300.11324751565675864No Hit
GCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAAA194110.10707489169085932No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCC191420.10559103481255108No Hit
CGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAA188300.10386998148157649No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCT185990.10259574007306645No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)