FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-046_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-046_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20462167
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC1948360.9521767660287398No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA1920460.9385418465209476No Hit
GTTTAATCAGCATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAAC1252350.612031951454604No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1110750.5428310696516161No Hit
CTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAAT832110.4066578090189568No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA760630.37172504749863494No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC746020.36458504126175884No Hit
GCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAAA690080.33724678329523944No Hit
CATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCT667720.32631929941731No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCT625960.3059109037669373No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA625920.30589135549524155No Hit
CGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAA619850.30292490526541005No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACAATGTTCCTCTAAGAAGCT618920.3024704079484837No Hit
CCCATCACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGA610060.29814046576787295No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCC598670.2925740954025055No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA576760.2818665295811533No Hit
CCCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCAT562490.27489268365369124No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT538960.2633934128286608No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT527070.25758268906709636No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC525500.2568154194030378No Hit
GACTAATTGAAGAGCAGGGAATAAAAATTCCCAACAACTTCAGTGAATTG517770.25303771589783236No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC514780.25157648258857435No Hit
CTTAAACTTCCTTGTGTTAGACACACAATGTTCCTCTAAGAAGCTTTTCA511680.250061491532153No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT489990.23946144120512747No Hit
GCCGTTCCCTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCA469860.22962377347423663No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC461310.2254453303992681No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATATA458780.2242089022145113No Hit
GGCACATATATAATTTCTCTTTTACATTAGCAGTAAAAAAGGAAACAAAA451010.22041165043761007No Hit
AAGAAACTCATAATACTATTGGCACAGGAATATCAACCTGTTTCCCTTTT445590.21776285962283468No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG440470.21526068084577749No Hit
GTACGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAA425710.2080473685900423No Hit
GGATTATGAATGCATCCGTAGAGGAAGCACGTATAATTTCTCTTTTACAT425480.20793496602779168No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCAATC413800.20222687069262998No Hit
CTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAAAAACG408890.1998273203419755No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC387950.1895938001092455No Hit
GGATTATTTATGTACCAAAAAAAGGCACATATATAATTTCTCTTTTACAT371420.181515476830973No Hit
CCATGCTATAGTATTCAAACACAGTAAATGCTTTAACTGTTTGCTTTGAA366340.17903284632561156No Hit
CACGAAAAAGGTGATAGGATTATTTATGTACCAAAAAAAGGCACATATAT362810.1773077113484608No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT356540.17424351976015054No Hit
CATCACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGAAA356130.17404314997526898No Hit
ATTGTTTAATCAGCATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCC351980.17201501678683395No Hit
GTCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAG347110.16963501470787526No Hit
GCACGTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAGA346420.16929780702112343No Hit
CCATTATCCAACAGACTTAGCTTCGCAACACTGGTTTGTCAACCAATTGC339490.16591106894983312No Hit
ATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCTT338980.1656618284857122No Hit
CACATATATAATTTCTCTTTTACATTAGCAGTAAAAAAGGAAACAAAAAA335660.16403932193496418No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTG333490.16297882819546922No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA331910.16220667146348672No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC319150.1559707727925395No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC316030.1544460076002703No Hit
CACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGAAAAGT315230.1540550421663551No Hit
GTAGGAGTACCACTATTCCCCGAAGGGAATTGGTGTAGTTTACATTCAGC313060.15299454842686017No Hit
AGCACGTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAG308280.15065852995921694No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA306650.14986193788761473No Hit
CTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACC304550.1488356536235874No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA304320.14872325106133677No Hit
CGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTATAA301720.1474526134011124No Hit
GCATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGC297070.1451801268164804No Hit
GTCAGTATCAACTCGATCCTCCATCAGACTTTCGTCTGACTTCAATCTCA296640.14496998289575097No Hit
CTGACATCTGAATACGAATGTCCCCAAGCTACTCCTATTAATCGTAACTA293900.14363092628459145No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA289510.14148550346598188No Hit
CTCACGAACAGCAGCTCACGAAAAAGGTGATAGGATTATGAATGCATCCG287030.1402735106208448No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC285640.13959420817941717No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAA282900.13825515156825766No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG279230.13646159764017174No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT278290.13600221325532139No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA277530.13563079609310197No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT273820.1338176938933203No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA271730.13279629669721685No Hit
CTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAAAAACGAAT268130.13103695224459855No Hit
TAAGAAACTCATAATACTATTGGCACAGGAATATCAACCTGTTTCCCTTT262820.1284419191769865No Hit
CTCGTTATATATATCAGTGTAGCACGCGTGCAGCCTAGTTCATCTAAGGA260920.1275133762714379No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA258560.12636002824138812No Hit
ATCACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGAAAA255920.12506984230946802No Hit
GTTCAATTTTGTTATTCCATGCTGTAGTATTCAAACGCGCAATGCTATAT252980.12363304433982969No Hit
CGCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTT251020.12267517902673748No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG246240.12033916055909424No Hit
CACGTATTAGTGCTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTC246040.12024141920061546No Hit
CTCTGACATCTGAATACGAATGTCCCCAAGCTACTCCTATTAATCGTAAC243980.11923468320828386No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT241170.11786141712165675No Hit
CGAGATAGTATTGTTTAATCAGCATGGTTTCTGGCTTAAGAGGCGTTCAG239560.11707459918590246No Hit
CTAAGAAACTCATAATACTATTGGCACAGGAATATCAACCTGTTTCCCTT237670.11615094334827783No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAAAATTT236220.11544231849930654No Hit
GTTCCCTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAA236010.11533969007290382No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT232270.11351192666935032No Hit
CTCGGCATGAAAAATTCCAATGAATAAATTAAAAAGAACTATGGGCCTTT229560.11218753126196263No Hit
GACCTGATGCGGTTGTTGGTACGCTCATACAAGTATTAAGAGTATAGCAA226130.11051126696405127No Hit
CCAAGGTTTGTTATAAAGGTAAAGTGCAAAGAAAACGCACCCCGAGTGTA226110.11050149282820337No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAC225560.1102327040923867No Hit
ACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCAC225500.11020338168484306No Hit
GTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGC225130.11002256017165729No Hit
AGCATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAG224950.10993459294902637No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG221430.10821434503979954No Hit
CTCTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTA220990.10799931405114618No Hit
GTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAGAACGA219510.1072760279984031No Hit
CCCCGTTCAAGAAATTTTTTGAATTGAGTTCAACGAACCTCTTCTCCAAG218810.10693393324372731No Hit
GGGCCTTTCACCCTCTATGGGGAAGCATTCCTGCTCACTTTGCTCAAATT218430.10674822466261762No Hit
GCACATATATAATTTCTCTTTTACATTAGCAGTAAAAAAGGAAACAAAAA218230.1066504833041388No Hit
CACGAAAAAGGTGATAGGATTATTTATGTACCAAAAAAAGGTACATATAT217050.1060738092891139No Hit
CCTGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA216230.10567306971935084No Hit
CGAAAAAGGTGATAGGATTATTTATGTACCAAAAAAAGGCACATATATAA211480.10335171245547943No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA211360.10329306764039214No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)