FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-028_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-028_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23543595
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA5855842.4872327272024513No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT4501381.9119340100779No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA4231351.7972403959548233No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC4023241.7088469284321277No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC3000531.2744570232371055No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA2540761.0791724883137006No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT2384741.0129039341697816No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT2347850.9972351291295999No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG2249560.9554870443532519No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT1616670.6866708334050089No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC1557470.6615259904020605No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT1505420.6394180667820696No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA1503680.6386790122748884No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA1453420.6173313803605609No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT1403450.5961069241974304No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC1265320.5374370396704496No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1219050.5177841361950033No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC1147820.4875296232372329No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA1128610.479370291580364No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC968690.41144523595483184No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA956120.4061062042564018No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT952480.4045601362068962No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA916360.3892183840233405No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA860290.36540298964537915No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG848170.36025509273328904No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG800950.34019868248668056No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA787060.33429898874831987No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT777460.33022144663973363No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA765730.32523919987580485No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG743860.3159500492596819No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA695370.29535421417162505No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA694270.2948869958050162No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT693020.2943560658429607No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC655360.2783602079461526No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA640420.27201453303966533No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA629840.26752074184082764No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC623590.2648660920305501No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA600140.25490584594238896No Hit
TCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAA597940.25397140920917133No Hit
TAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAAT595240.2528246004911314No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC580050.24637273959223302No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTC577710.24537883870326518No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC574330.24394320408586712No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTATC560130.23791183971691665No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT554400.23547805677085423No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA548140.2328191595208803No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG532730.22627385494866015No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC532070.22599352392869482No Hit
GCCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGAT527820.22418836205770615No Hit
GCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGA525320.22312650213359514No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT525260.22310101749541647No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG524580.2228121915960583No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC509780.2165259808453212No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA507410.21551933763726397No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC484560.20581393793088948No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT476590.20242872849282365No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT464410.19725534694255484No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT457230.1942056852405081No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA455750.19357706416543438No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT449500.1909224143551569No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC423220.17976014283290212No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC419020.17797621816039563No Hit
CGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAA413430.17560189937008344No Hit
TCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCC410370.17430218282297158No Hit
CGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTATAA404290.17171973948753366No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT401590.17057293076949379No Hit
CCCACTTGGAGTTATATTATTTGACAAACGGGCAAAATACAAATAAAGAT393210.16701357630387373No Hit
GGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAAT390010.16565439560101164No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA389110.16527212602833172No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA381110.1618741742711765No Hit
TACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA372920.1583955211597889No Hit
GTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAAT370090.15719349572569524No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG365680.15532037481956346No Hit
CATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCT363570.15442416504361378No Hit
ACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT359020.15249157998173177No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA355090.15082233618102928No Hit
TGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAATA354970.15077136690467194No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG335970.14270123148142838No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG335130.1423444465469271No Hit
CCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC330510.14038212940716996No Hit
CGGCACAGGAATATCAACCTGTTTCCCTTTCGAGACGGGGATGAAATGCA327850.13925231044791586No Hit
GTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATCTCG316760.1345418998245595No Hit
CGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAA316740.1345334049451666No Hit
GGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATC313120.1329958317750539No Hit
TCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCT311340.13223978750908685No Hit
ACGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGA309950.13164939339128115No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC287590.12215211823003241No Hit
GCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCA285190.12113273270288585No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG282420.11995619190697088No Hit
CACGTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATC281360.11950596329914781No Hit
CTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAAATAGTCCA277980.11807032868174974No Hit
CAAAAAAATAATTTGCCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAA274340.11652426063224414No Hit
CCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAA266590.11323249486750006No Hit
AATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAA266420.11316028839266051No Hit
ATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCTTTC264610.11239150180760414No Hit
TCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC260580.11067978360993723No Hit
CGCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTT260210.1105226283411688No Hit
ATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCTA258930.10997895606002397No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC256830.10908699372377073No Hit
GTGGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAA253280.10757915263153311No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT252850.10739651272458602No Hit
CTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCTT249480.10596512554688439No Hit
TCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAA248590.1055871034139009No Hit
GCCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGT248540.10556586621541866No Hit
GAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAATAC248180.10541295838634669No Hit
AGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAG247270.10502644137397028No Hit
GTCAGAAACTCGATTGATACACACTAAAAATGGACGGATCCATTACGTGT246630.10475460523339786No Hit
GCACCCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTA243350.10336144501296425No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAAGA241780.10269459698062254No Hit
CCCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC237660.10094465182568763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)