FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-023_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-023_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43514494
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAATTGAGTTTTGGGAAGGTTTTAAATTCCCATCATTCCAATTACAA5741261.3193902702855742No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA5292321.216220048428002No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC4880361.1215481443952906No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC3861380.8873778929843469No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT3703960.8512014410646714No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA3646400.8379736645909291No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT3490720.8021970794374857No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC3190620.7332315526867899No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT3189880.733061494406898No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG2871820.659968607241532No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA2850810.6551403309435242No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC2719920.6250606981664546No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC2594750.596295569931251No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT2582620.5935079929919442No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT2560580.588443013953006No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC2494030.5731492591870654No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA2403560.5523584854278668No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC2395980.5506165371014081No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA2291320.5265647809210421No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT2212760.5085110262341554No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT2200220.5056292278154493No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA2168530.4983465968833281No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC1996680.45885400850576363No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG1962730.4510520103945136No Hit
CCCTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACG1941950.4462765900483641No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA1847280.4245206206465368No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA1665210.3826793895385754No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA1638190.37646996423766294No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC1636980.37619189596919134No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA1554580.3572556766947583No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA1549300.356042287886882No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG1445540.3321973593442222No Hit
CATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCG1386190.3185582256799309No Hit
CATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAA1353560.31105957477065No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC1350700.310402322499717No Hit
CTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGAC1310450.30115253092452365No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA1308270.30065154842430203No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT1308020.30059409630271694No Hit
CAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGTAA1286410.2956279349129051No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA1265730.29087549541538965No Hit
CACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAAAT1244620.2860242382687479No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG1243840.2858449876494025No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC1195080.27463952585545404No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1178900.2709212245464695No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC1076150.2473084025750133No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT1063070.24430250757368335No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT1056930.24289148346755451No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGAAAA1033060.23740595489861377No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA1030540.23682683751303646No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA1026810.23596965185898747No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT1021240.23468961859007256No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA1002940.23048412329004678No Hit
CCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCG951700.21870873644997457No Hit
CCACGATTTTCTACTCATAAAAGGATACTAAAAAGAAGAAGTTCTTTTCT951110.21857314944303385No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG934570.21477211707896685No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT919900.211400826584356No Hit
CACGATTTTCTACTCATAAAAGGATACTAAAAAGAAGAAGTTCTTTTCTT909920.20910733789068076No Hit
CCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCT897930.20635193413946168No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG897680.20629448201787662No Hit
CCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTC890000.20452955284278382No Hit
ATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAA888170.20410900331278128No Hit
CTCCACTTCAGTCTTCAAAGTTCTCATTTGAATAGTTGCTACTTCCACCA870850.20012872032936888No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT864310.19862577282870392No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA852090.19581751312562662No Hit
CGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGA847930.19486150982245135No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC845570.19431916179468844No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA820690.1886015266545441No Hit
CGCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTT806850.18542097720359565No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT792640.18215539861270133No Hit
CCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAA788930.18130280912837915No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA787560.1809879715020931No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT782540.17983433290066525No Hit
GTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAGTGAA779530.17914260935678122No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA777000.17856119388634048No Hit
GCTACTTCCACCAAGATCTGCACTAGGGATATGTTCCACGAAAACTCACG768810.1766790623832142No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC749480.17223686434225802No Hit
GTAAAATTATATTAGTCAACTCCAGCGATCCTGAGAGATCTTTCGAAAAT747130.17169681439935852No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC733180.16849098601491264No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC729520.16764988695490748No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA714560.16421195199925798No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC675940.15533674825679922No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT671630.15434627368067294No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG659630.15158857184459046No Hit
CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCC657350.15106460849573478No Hit
CCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAG654980.1505199623831085No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG653530.1501867400779152No Hit
GCTCCCTCTCCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCAT651650.1497547001235956No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT634070.14571466693373478No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT628890.1445242589744925No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC617610.141932019248575No Hit
CCTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGA605670.13918810592167288No Hit
CTCCCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTT600470.13799310179270383No Hit
ATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGT596410.13706007933816258No Hit
CACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCTT591930.13603053731935846No Hit
CCTCACGGTACTTGTTTGCTATCGGTCTCCTACACATATTTAGCTTTATG586950.13488609105738425No Hit
CCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAA571550.13134704036774505No Hit
CCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTG566810.13025774814249247No Hit
TACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA563850.12957751502292547No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC560030.12869964660510588No Hit
CTTCCGTCAATTCTTTTAACTTTCTCGCTTGCGCGAATACTCGCCCCAGA559990.1286904542656523No Hit
CCCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTG555560.12767240267116514No Hit
ACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGTAT529230.12162154522582752No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA524550.12054604150975536No Hit
CGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGGCT523060.12020362686510844No Hit
ACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT511710.11759530054514708No Hit
GCCACTTATTAATTCAATTAATATGAATACCCGGCGAACCGAGTAATATA511670.11758610820569348No Hit
CTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGTATGCGCCGACAG508030.11674960531541513No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA507620.11665538383601566No Hit
CGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAA503050.1156051590534409No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGGAAGATGAATT500470.11501225315868319No Hit
GGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTA498140.11447679938551049No Hit
TTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGCA497500.11432972195425276No Hit
AGGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTA492000.11306577527938162No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT489910.11258547554293058No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC485010.11145941395986358No Hit
CAGCGATCCTGAGAGATCTTTCGAAAATAACCAGCTAATAGGCTTTTCGA479780.11025751557630431No Hit
CTCCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGC476980.10961405181455172No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGGAAGATAAATT476400.10948076289247441No Hit
ATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCTTTC474990.10915673292673472No Hit
CGGTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAG469860.10797781539180944No Hit
TCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAA461830.1061324532464976No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAACATAGCGTAATTGCTTACC461140.10597388539092284No Hit
CCCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGG456940.10500868974829397No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC451700.10380449327987129No Hit
CCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACA451240.1036987813761548No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC446470.10260259489631202No Hit
TCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCC441510.1014627448040646No Hit
GTACAAAGGGCAGGGACGTAGTCAGCACAATCTGATGAATCATGCTTACT436040.10020569238378366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)