FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-020_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-020_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42115310
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT5552801.3184753952897414No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT4776801.1342193610827036No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC4412381.047690258008311No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC4172640.9907655909454305No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA3345100.79427172683758No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC3166640.7518975878368223No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG2926110.6947853405329321No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA2739690.650521152521494No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA2530680.6008931193905495No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC2530350.6008147630873428No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA2494900.5923973965762095No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAA2423040.5753347179446144No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA2285680.5427195003432244No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC2184180.5186190010236182No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA2001740.47529983751752036No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT2000740.47506239417447005No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT1995940.4739226661278286No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1993250.47328394353502323No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT1969100.46754968680035836No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT1952780.46367461144177735No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT1776400.42179435459456427No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA1774480.4213384633759077No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT1580270.3752245917221077No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA1502610.3567847417008209No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG1501930.3566232802275467No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA1499640.3560795349719615No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA1442690.34255713658524656No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAAAAAAAAGAA1411230.33508716901288393No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC1406500.33396406200025597No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA1388550.32970195399250296No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC1379310.32750797750271815No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT1362610.32354267367377804No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC1259030.2989482922006273No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC1244590.2955196103269809No Hit
GCACCATGTAGGAACAACTAAAACAGCAAAGAAATAAATCAATGCAATTT1129820.2682682378450972No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC1123290.2667177328149787No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA1095240.26005744704241757No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT1057040.25098711133789586No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA1017280.2415463640182157No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT998150.23700407286566336No Hit
GCCACTTATTAATTCAATTAATATGAATACCCGGCGAACCGAGTAATATA992730.2357171299463307No Hit
CCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCT989720.23500242548374928No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT985730.23405502654497853No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT976310.23181831025344463No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT972340.2308756601815349No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG968540.22997337547794378No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC955530.2268842375848593No Hit
GCCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGC913370.21687362624185835No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC884880.21010886539835513No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA860730.20437460866369025No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA837720.19891103734010268No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA821300.19501221764721666No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC819860.1946702992332242No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA816770.19393659930319876No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT794480.18864398718660744No Hit
CTCCAATTGAGTTTTGGGAAGGTTTTAAATTCCCATCATTCCAATTACAA794320.1886059962517194No Hit
GTAAAATTATATTAGTCAACTCCAGCGATCCTGAGAGATCTTTCGAAAAT784280.1862220650874943No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG781360.18552873052578742No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA780240.18526279398157106No Hit
CATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAA779190.18501347847136826No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT765760.18182461437420264No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC763980.1814019652235731No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC732050.1738203992799768No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT729500.17321491875519854No Hit
CCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCG716540.17013765302926656No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT707050.16788431570371914No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG703670.1670817572042091No Hit
GCCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGAT694960.16501362568624092No Hit
CGCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTT691520.16419682058614787No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG675570.1604095992644955No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA673850.16000119671444896No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC671570.15945982589229427No Hit
CTCCCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTT665920.15811827100406006No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC663000.15742493644235314No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA653570.15518584571738878No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATGTA650530.15446401795451584No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT649230.15415534160855043No Hit
GTCAGAAACTCGATTGATACACACTAAAAATGGACGGATCCATTACGTGT647620.15377305782623943No Hit
GTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATCTCG637340.15133214025968228No Hit
CATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCG596410.14161358422863324No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG582510.138313121760234No Hit
GTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTTTCCCGCT582100.13821576998958335No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA581660.13811129491864124No Hit
GCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCA580090.13773850887005223No Hit
GCCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAA579940.1377028923685947No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA562930.133663981103309No Hit
GTTGGTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAA557090.1322773119798952No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG556490.132134845974065No Hit
GGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTA556140.1320517408039974No Hit
CACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCTT551690.13099511792742355No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA551100.13085502635502386No Hit
GGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATC546550.12977465914414496No Hit
AGGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTA544470.12928077699060034No Hit
CTCGTTATACATATCAGTGTAGCACGCGTGCAGCCTAGTTCATCTAAGGA543750.1291098177836041No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGGAAGATAAATT537170.1275474405863331No Hit
GCACGTATAATTTCTCTTTTACATCAGCAGTAAAAGGATAACAAAAAAGA532220.12637209603823407No Hit
CCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAG529880.12581647861549636No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT524840.12461976416652282No Hit
CATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCT522090.12396679497313447No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG509990.12109373052222577No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC502240.1192535446135859No Hit
GTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAGTGAA496300.11784313115586707No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACAACGCC492240.11687911118308282No Hit
GGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAAT485380.11525024984975774No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG483240.11474212109563006No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT469250.11142028872635629No Hit
CCCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGG461910.10967745458836703No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA459140.10901973652811768No Hit
TCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCT457240.10856859417632211No Hit
GTGGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAA456840.10847361683910199No Hit
CGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAA456480.10838813723560387No Hit
TCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCC447650.10629151251646968No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC445870.10586886336584013No Hit
ATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAA436770.10370812894408234No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTC433590.10295305911318235No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC430270.10216474721425535No Hit
CGTCATTTTGCAAATATAAAGGATCGATAGGCCACGCTTTCACGTTTTCT428960.10185369643485945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)