FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-014_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-014_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14725409
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT1380190.937284662178144No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC1310060.8896594994407286No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA1283820.8718399604384502No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC1061760.7210393952385296No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT930270.6317447617244452No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA844850.57373618620712No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT735300.4993409690691783No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC699600.4750971602894018No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG656150.4455903397997298No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT569140.38650199800901963No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA503120.3416679292235618No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC462250.31391318230957116No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC461690.31353288726988837No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAACAGAA416730.28300062836964324No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT412580.2801823704862799No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA410720.278919247675905No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT408840.2776425428998271No Hit
CCTATGTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTC407780.2769226987175704No Hit
CATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCT384700.26124911029635917No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC356250.24192876408390424No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA348500.23666575237400878No Hit
TCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCC339550.23058782272193595No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA320410.217589881544207No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA312960.2125305993198559No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT296990.20168539970604554No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT289630.1966872363273577No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT287790.19543769548268577No Hit
TAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAAT286480.19454807672914212No Hit
CCAAGGTTTGCTATAGAGGTAAAGTGCAAAGAAAACGCACCCCGAGTGTA276440.1877299299462582No Hit
CGGCACAGGAATATCAACCTGTTTCCCTTTCGAGACGGGGATGAAATGCA265310.18017156603256318No Hit
TGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAATA259570.1762735418758148No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA254540.17285767750152137No Hit
GTTTAATCAGCATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAAC242250.16451155957705488No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA240030.16300396138402676No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA238190.1617544205393548No Hit
GTGAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAAT237700.16142166237963237No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC235490.15992085516945573No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA224000.1521180158731075No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT223640.1518735404904543No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG223350.15167660198776142No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT218460.14835581137338869No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG217360.1476088032597261No Hit
GGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAAT209600.14233900056697915No Hit
CAAAAAAATAATTTGCCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAA206790.14043073438571382No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC205670.1396701443063483No Hit
CGAGATAGTATTGTTTAATCAGCATGGTTTCTGGCTTAAGAGGCGTTCAG204580.1389299271755372No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC202050.13721180851411324No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT198070.134508997339225No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC194690.13221364513542544No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCATGTAT193950.13171111240441608No Hit
CACGTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATC192600.13079432971946653No Hit
GTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATCTCG190790.129565161823349No Hit
CCTAAACGTGACTGAAATGAACGATTTGCACGTCAGTATCAATTCGATCT186790.12684876868275782No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC185570.12602026877487751No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT182280.12378603541674123No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA180190.12236672000078232No Hit
CAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCTTTCGAGACGGG179910.12217657248094094No Hit
CGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTATAA179820.12211545363527763No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG179110.1216332938528227No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA178630.12130732667595176No Hit
CTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCTTTCGAGACG177950.12084553984205125No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA174560.1185433966554002No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTTATGAAATAATTA174490.11849585977543986No Hit
GAAGGAGTTCATTTGTATATATGTTACCATCTCTATTACACTCAAAATAC174300.11836683110126177No Hit
CCATTATCCAACAGACTTAGCTTCGCAACACTGGTTTGTCAACCAATTGC169900.11537879864661144No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG169210.11491022082985947No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA168550.11446201596166192No Hit
GGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATC167780.11393911028209812No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA166600.1131377743056237No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC165260.11222778260352566No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA159400.10824826665255954No Hit
CGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCATGTATAA159240.10813961092693589No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTTATGAAATAA156760.10645544717976935No Hit
CTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAAATAGTCCA155920.1058850046202452No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC154500.1049206850553353No Hit
GTGGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAA151700.10301920985692145No Hit
CGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAA151390.10280868938852565No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA150800.10240802140028843No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC149730.10168138623518029No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT149390.10145049281823004No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)