FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-010_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-010_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18223747
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT1016400.557733818407378No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC696640.3822704518450569No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA661000.36271355171908387No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC635250.34858363650461127No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT620400.34043492811878917No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG558770.306616416481199No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC466350.2559023673890995No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA421950.23153855241734864No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA388520.2131943556942488No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT328610.18031966751952822No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA321650.17650047490233484No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT304620.16715552515078266No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT265550.14571646544478475No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA262570.14408123642190598No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT258400.14179301325901858No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT255430.14016327158185418No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA249860.13710682001895658No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC217180.11917417422443363No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT213790.11731396402726618No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAA206930.11354964486721639No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC202370.111047415221469No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG193650.10626244975854855No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA193300.10607039265854601No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT190510.10453942320424005No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA188070.10320051084993663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)