FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-008_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-008_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25556808
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC1444780.5653209900078288No Hit
CTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAAC551300.21571551502049865No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC517890.20264267744234724No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG481500.18840381005327425No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGGCCC465890.18229584852693653No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC431300.16876129444647392No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC429160.16792394417957046No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT411100.16085733398317975No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC364880.14277213335875122No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA346290.1354981420214919No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC335880.13142486338669523No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGGGCC318380.12457737288631664No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC298620.11684557789846056No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG296180.11589084208012207No Hit
CGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG285910.11187234336932844No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC266620.10432445241205396No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG263150.10296669286712175No Hit
CCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCG260590.10196500282820921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)