FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_MHC-004_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_MHC-004_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33915902
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCAT3393421.000539510935018No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCT2455120.7238846249762132No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC2422110.7141517274109355No Hit
CTCCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAAT2299040.677864914222243No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC2191900.6462750128243677No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCC1804770.532130916052299No Hit
CTCCAATTGTTACTCTGGGAAGGTTTTAAATTCCCACCATTCCAATTACA1712710.504987306544287No Hit
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA1530950.4513959263120881No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA1464880.4319153888344175No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC1425430.42028367696073665No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1420450.4188153391880894No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT1400220.41285058554538817No Hit
CGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAA1335600.3937975761340506No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAAATTGGGAGAAA1180920.3481906510992985No Hit
GCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAAGAAA1165530.34365295665732254No Hit
GTACGCTCATACAAGTATTAAGAGTATAGCAAAGCAAAATGGAATCAGAA1097980.3237360457050501No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTG1072790.31630885122854757No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGACAAATGCTTTCGC1023870.3018849388112986No Hit
GCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTTC998810.294496074437295No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC955300.28166728397788154No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC950450.2802372763077332No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT915450.2699176333272811No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC901640.265845797054137No Hit
CCGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATC901210.2657190128689486No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA866100.25536693672484373No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT857490.2528283045516525No Hit
CGCCCTTTTTACCTATTGGGAGTTTGAGAATAGGTCAAGGAAAACGATTT851430.2510415320813228No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGTAAGTAATTTA847080.24975894788232378No Hit
CACCATTCCAATTACAAAACCAAAAATTGGCCTTGCATTGTTATTTCTTG825310.2433401299484826No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC792750.23373991350723916No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA777840.2293437455975666No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC760070.22410431543291995No Hit
CCCATCACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGA735680.21691299851025633No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC734120.2164530372802705No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC725720.213976322964962No Hit
CCCTTGTCCGTTTCAGTTCTGAGTTAACATTATAGGTATCAAATTAAAGT711480.20977770250662947No Hit
CTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACC661280.19497638600323824No Hit
CTCCACTTCAGTCTTCAAAGTTCTCATTTGAATAGTTGCTACTTCCACCA655920.19339600639251758No Hit
TCCAATTGTTACTCTGGGAAGGTTTTAAATTCCCACCATTCCAATTACAA623120.1837250266851225No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT596980.17601772761343631No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATAGGTATCAAATTAAAGTAATAA592850.17480000974174298No Hit
CTTCCGTCAATTCTTTTAACTTTCTCGCTTGCGCGAATACTCGCCCCAGA562620.1658867866760554No Hit
AGGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTA550400.1622837570411661No Hit
GTAAAATTATATTAATCAACTCCAGCGATCCTGAGAGATCTTTCGAAAAT544900.16066209885852364No Hit
CCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGACAAATGCTTTCG527830.15562906155348605No Hit
CTCGGCATGAAAAATTCCAATGAATAAATTAAAACGAACTATGGGCCTTT525320.15488899572831646No Hit
GCCGTTCCCTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCA520170.15337053397547853No Hit
CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCC516980.15242997222954588No Hit
CTCCCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGACAAATGCTT516250.1522147339616679No Hit
CCACGCTTTCACGTTTTCTACTTGTTCTGAAAATAGAAATCAAACAAGCT512420.15108547017266416No Hit
CTCCTTTTTTCAAGGATAGTCCTTACCACTTCGGATAATTCATAAAAACG511710.1508761288436321No Hit
CCCATCACGATATATATTGATAAAGATTACCTACACCTTTCGGCGGAGGA508130.14982057679020302No Hit
CCAATTGTTACTCTGGGAAGGTTTTAAATTCCCACCATTCCAATTACAAA496990.14653598185299627No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG495450.1460819175618564No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC486170.14334573793732508No Hit
CCCTTACCTACTTTTTTCTATTTAACCAGAGGCTGTTAACCTTGGAGACC481310.14191278179775377No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC470000.13857806288035623No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCAATC459020.13534064345391728No Hit
GCCACGCTTTCACGTTTTCTACTTGTTCTGAAAATAGAAATCAAACAAGC450200.13274009342284337No Hit
CGAGTGTAAAGCCTATGTAAAGGAGTTCATTTATATATATGTTACCATCT447590.13197054290344393No Hit
CCCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGTAAGTAATTT447490.1319410582092141No Hit
GTCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAG427890.1261620581401609No Hit
GTACAAAGGGCAGGGACGTAGTCAGCACAATCTGATGAATCATGCTTACT427200.12595861374997486No Hit
CATCACGATATATATTGATAAAGATTACCTACGCCTTTCGGCGGAGGAAA417900.12321653718659761No Hit
CCTCACGGTACTTGTTTGCTATCGGTCTCCTACACATATTTAGCTTTATG416780.12288630861122314No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC412300.12156539430972528No Hit
CTTGTCCGTTTCAGTTCTGAGTTAACATTATAGGTATCAAATTAAAGTAA411070.12120273257069794No Hit
CCTCGTTCAAGATTAATAATTGCAATAATCTATCCCCATCACGATATATA406250.11978157030881856No Hit
GTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTACCGGTAT401680.11843411978251382No Hit
CCGACATCGAAGGATCAAAAAGCTACGTCGCTAGGAGCGCTTGGCAGCCA393710.11608418965239373No Hit
GCCCTTCCGTCAATTCTTTTAACTTTCTCGCTTGCGCGAATACTCGCCCC383820.11316815339306029No Hit
CCCCGTTCAAGAAATTTTTTGAATTGAGTTCAACGAACCTCTTCTCCAAG381050.11235142736289308No Hit
CCTTCCGTCAATTCTTTTAACTTTCTCGCTTGCGCGAATACTCGCCCCAG379680.11194748705194396No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA375560.1107327176496736No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC374760.11049684009583469No Hit
CCCACTTGGAGTTATTTTATTTAATATGAGTGAAATACAAATTGAGTTAT372740.10990124927239145No Hit
CCAGAACCCAAAGACTTTGATTTCTCATAAGGTACTGAAGGAAGCAATCT368640.10869237680896707No Hit
CCACGATTTTCTACTCATAAAAAGATTCTAAAAAAAAAAATAAATTTTTT368440.10863340742050735No Hit
CTCTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTGCTTA367120.10824420945667315No Hit
CCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAG363960.10731249311900948No Hit
CCAATCTCAAAGAACACGACTCGGCATGAAAAATTCCAATGAATAAATTA363860.10728300842477961No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGTATTAGTGCTCCA361640.10662844821287666No Hit
CTCGGGTCCTACCAAATATGCTCGTACTCAATAAAGCATAATTTTGCTTA360730.10636013749538492No Hit
CCCCTCTTTACTTGTTTTCTCTCTTAATCCGTTCCATCTTAACGATTTCA355650.10486231502850787No Hit
GCTACTTCCACCAAGATCTGCACTAGGGAGATGTTCCACGAAAACTCACG351820.1037330512395041No Hit
GCCTAGTTCATCTAAGGACATCACAGACCTGTTGTTGCCTTAAACTTCCT343800.1013683787622691No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG342710.1010469955951636No Hit
CAGCGATCCTGAGAGATCTTTCGAAAATAACCAGCTAATAGGCTTTTCGA341860.10079637569420975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)