FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_KHC-009_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_KHC-009_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18910431
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA3420461.8087689275828773No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT2262841.1966094268290342No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC2261231.1957580448589458No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG2201911.164389114134945No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC2167341.1461081981685135No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT1877180.9926690724288623No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC1836270.9710355094497846No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT1673670.8850512185576309No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA1605480.848991754867988No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA1439220.7610720242177452No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC1421070.7514741467288609No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAA1252920.6625549676789492No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA1128740.5968875061599601No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA1105300.5844922307693569No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT1010060.5341284923648753No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT1008540.5333247031757236No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT976980.5166355013272833No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA906960.47960831775859575No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA854840.45204680950952414No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA838770.4435488540689527No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT806400.42643131719208305No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC787830.4166113400588279No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA766520.4053424271503912No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA763630.4038141700736487No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT755180.3993457367523776No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA749200.396183460863478No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA741510.3921169221367826No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT740570.39161984198033356No Hit
CCCCTTTTGAGAATAGTTATAAAACCAGTTGTCCCCTCGTTAGTGTGGGG735070.38871139425642914No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGATA733710.3879922144556092No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT712770.37691896075768977No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG643650.3403677049983684No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA641560.3392624948632847No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC602030.31835868785856863No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA581040.30725899372679555No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG561120.2967251248794911No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT546880.2891948893179642No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT542000.2866143029738455No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC536010.28344673899817513No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG520200.27508627381364287No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT501180.2650283327757046No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC495590.26207229227086365No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA486480.2572548452227239No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT474270.2507980912756563No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC466090.2464724362971949No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA462660.24465862253483273No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT444610.23511362591365578No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC443330.23443675080700171No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC415990.2199791215758118No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC415280.21960366741508958No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA404090.21368629831863695No Hit
GCCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAGCGAAGAT392830.20773191261478913No Hit
GTTAAGAAAGACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAA390580.20654209309137378No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG389860.20616135084388082No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA387820.20508258114265085No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC370130.19572795564522036No Hit
CCCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC349140.18462826151344724No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT335690.1775157848068085No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT333940.1765903696219298No Hit
GGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTA332060.1755962093090316No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG327550.17321128217543005No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC326700.17276179479991757No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC315440.16680740909606978No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA312480.16524213541193217No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC309190.16350235486436032No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC307410.16256107541916945No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA304190.16085831147899274No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA287010.15177337840686975No Hit
TTTTGAGAATAGTTATAAAACCAGTTGTCCCCTCGTTAGTGTGGGGACTA282690.14948892492191213No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC279770.14794480358485748No Hit
CACGAATTAGTGCTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTC276900.1464271226816565No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT273350.1445498518780455No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT270500.143042747148386No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC265690.14049917741166237No Hit
TTTGAGAATAGTTATAAAACCAGTTGTCCCCTCGTTAGTGTGGGGACTAA260310.13765418672900687No Hit
CGCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTT257380.13610477730518147No Hit
CCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCT256880.1358403729666447No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG255480.1351000408187418No Hit
CTTTTGAGAATAGTTATAAAACCAGTTGTCCCCTCGTTAGTGTGGGGACT255440.13507888847165886No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG255210.13495726247593196No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG248630.1314777013807882No Hit
CCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTG247340.13079553818736336No Hit
CATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAA246960.13059459089007544No Hit
GTCAGTATCAATTCGATCTTCCATCAGACTTTCGTCTGACTTCAATCTCG246610.13040950785309968No Hit
CCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAA246520.1303619150721631No Hit
TTAAGAAAGACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAAT241230.12756451717044417No Hit
TACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA240530.1271943510964927No Hit
AATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAA239670.1267395756342095No Hit
GCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCA239070.1264222904279654No Hit
TCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCT236100.12485172865705704No Hit
GCACCCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTA232670.1230379148946949No Hit
CGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAA232540.12296916976667534No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG231950.12265717264720195No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG231190.12225527805262608No Hit
GTTGCCTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCT225110.11904012129601911No Hit
TCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCC222190.11749599995896445No Hit
ATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCCTTTC218080.1153225962961923No Hit
CTTAAACTTCCTTGTGTTAGACACACATCGTTCCTCTAAGAAGCTTTACA217470.11500002300317744No Hit
ACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAAT216520.11449765475995761No Hit
CACGAAAAAGGTGATAGGATTATGAATGCATCCGTAGAGGAAGCACGTAT215290.1138472200871572No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAAAG215190.11379433921944984No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGGAAGATAAATT212750.11250404604739045No Hit
CGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGA206400.10914611094797365No Hit
CATGGTTTCTGGCTTAAGAGGCGTTCAGCCATTATCCAACAGACTTAGCT205440.10863845461798306No Hit
CCCTTTTGAGAATAGTTATAAAACCAGTTGTCCCCTCGTTAGTGTGGGGA202660.10716836649571869No Hit
CTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAAATAGTCCA198870.10516418160961007No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC197340.10435510433368757No Hit
CCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC195650.10346141766943333No Hit
TCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTAC194690.10295376133944276No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT193840.10250427396393028No Hit
ATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCTA189380.10014578726418238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)