FastQCFastQC Report
Wed 17 Jan 2024
HNTFHDMXY_n01_KHC-008_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTFHDMXY_n01_KHC-008_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27820450
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAA5613052.0175985650843176No Hit
CCTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTT4624371.6622196980997792No Hit
CTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGG4586481.6486002203415113No Hit
CTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTC3743251.345503038232667No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAA3424161.2308068345407783No Hit
CCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGA3226271.159675706180166No Hit
CTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCT3140071.1286913044181528No Hit
CCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCC3056981.0988247853647226No Hit
CTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACAT2781070.9996495383791419No Hit
CTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACC2429130.8731454739229596No Hit
CCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTA2376420.8541989795276496No Hit
CCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGT2364340.8498568499071726No Hit
CTCCAATTGAGTTTTGGGAAGGTTTTAAATTCCCATCATTCCAATTACAA2308910.8299326574516229No Hit
CCCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGC2187520.7862992870352563No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAA2051790.737511434933655No Hit
TTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACATC2041230.7337156659939001No Hit
GCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAATGCAATTT1986880.7141796771799163No Hit
CTGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTT1753040.6301263998245894No Hit
GTACGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAA1678090.6031857859955536No Hit
CCCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCAT1669400.6000621844722138No Hit
GGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTAC1630520.586086853375844No Hit
GTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCT1580140.5679778723924308No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGCAAAATTCCAAAAAATAGAA1574080.5657996186258669No Hit
CCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGA1507480.541860394062641No Hit
CGCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTA1502780.5401709893261971No Hit
CCCCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTT1502600.5401062887192695No Hit
CCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCT1492630.5365225939911109No Hit
CCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTT1492250.5363860038209303No Hit
CCCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCT1490540.5357713480551177No Hit
CCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGAC1463710.5261273631447371No Hit
CTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCCC1444520.5192295595506183No Hit
GTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGACGCC1333190.4792122341658744No Hit
CCATGCTGTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACA1329180.47777084842265316No Hit
CTCTCGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTA1290770.4639644577999278No Hit
GTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTG1233640.4434292040567281No Hit
CCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGA1196570.4301044735077973No Hit
TACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCA1188280.4271246511109633No Hit
CCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCG1143110.4108883932502889No Hit
CCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTC1094630.39346236311777844No Hit
GTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTAT1079440.38800235078871836No Hit
AATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCT940550.33807864358772055No Hit
CATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCG927710.3334633336268824No Hit
CTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCGC899310.3232550156449662No Hit
GCTCATACAAGTATTAAGAGTATACAAAAAAAGGTTAATGAAATAATTAA893710.32124210787388413No Hit
CACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAATC890230.3199912294732832No Hit
CTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGT865880.3112386751472388No Hit
GAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGC836150.30055229156969065No Hit
CCCTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACG815100.2929859150373197No Hit
CCGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAA814620.29281338008551266No Hit
GCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAAT787840.2831873675659452No Hit
TTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGA764680.2748625561412558No Hit
CCGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTG753640.270894252249694No Hit
CCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAAC747380.2686441089198773No Hit
GCCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAA734100.2638706419198827No Hit
GTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTACTC717850.258029614905582No Hit
CTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCA714400.25678951993946897No Hit
GCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTACC706020.25377734723917117No Hit
CCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGATAGGT705490.2535868398965509No Hit
CGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAG691590.24859051525047224No Hit
CCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAG690800.24830655147562317No Hit
GCCACTTATTAATTCAATTAATATGAATACCCGGCGAACCGAGTAATATA684730.2461247032308967No Hit
CATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTT633350.22765627443121877No Hit
CTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATT633030.22754125113001408No Hit
GGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTA627950.22571525622338962No Hit
CCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGGAAGATGAATT617910.22210640014809252No Hit
CATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAA609510.2190870384914694No Hit
CTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACG609400.2190474992316803No Hit
GGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAAATTAAGA596230.2143135714914748No Hit
CCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTC586000.21063642033108737No Hit
CCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCATGTTAGGCCA570310.20499668409389493No Hit
CCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACA567150.20386082899449864No Hit
CAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGTAA566000.2034474640057943No Hit
TCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTCC553440.19893279943351025No Hit
GTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCA541530.19465177594179822No Hit
TCCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGG540670.19434265081981061No Hit
GTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGC540100.19413776556453977No Hit
CTCCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAGAAATAGAA535080.1923333375268912No Hit
CCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGGACA504380.1812982895675663No Hit
CCCCAGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGAAAA498880.1793213265781107No Hit
GTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAA492110.176887864861999No Hit
CCCCCTTTTAGTCTACAAGAGATTTCTGTTCTCTTTGAGCTTGTCTTTGG487670.17529191655778392No Hit
TGAATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTG484140.17402306576636972No Hit
CCCCATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTG475550.17093540902465632No Hit
GTAGTATTCAAACGCGCAATGCTATATCTGTTTGCTTTGAACACTCTAAT470100.16897641842601396No Hit
CTTGTCCGTTTCAGTTCTGAGTTAACATTATTAGTATCAAGAGGGACGAC466870.16781540197947914No Hit
CCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGGTATTG464240.16687005422270307No Hit
CCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAAC463730.1666867358364081No Hit
CCCCTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGG444490.15977095985147619No Hit
CTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAGAACAAAATTCTG438900.15776164655855676No Hit
GTCGCTTCTTTGTTATAATTCCTTAAGTTAATAACTTTTTTCTTAAAAGG438880.15775445760223145No Hit
CCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGCT432260.15537491305855944No Hit
CCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTTTCG431690.1551700278032886No Hit
GTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAGTGAA425920.15309601390344152No Hit
CCACGATTTTCTACTCATAAAAGGATACTAAAAAGAAGAAGTTCTTTTCT423270.1521434771903402No Hit
GGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATC420620.15119094047723886No Hit
GTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAGCTCAA417260.14998319581458963No Hit
CGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGAA413040.14846632602995277No Hit
AATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAA401890.14445848287860188No Hit
GGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAAT399820.14371442589893407No Hit
CGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAG395210.14205737146595399No Hit
AGGCACCTTATCCAAGCGTTAGGAGCATCCCTCATCGCCAGTTCTGCTTA391050.14056206855029305No Hit
ATCAATAGATAAGTAAAACAACTTTAAAAGTAGTGGTATTTCACTGTCGT390640.14041469494562453No Hit
CCGAGTGTAAGACCTATGTGAAGGAGTTCATTTGTATATATGTTACCATC386040.13876123499080711No Hit
ATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAA372360.13384398886430665No Hit
CTCCCTTAACTTTCGTTCTTGATTAATGGAAGTATTTTAGGCAAATGCTT372230.13379726064819225No Hit
CCTTGTTTCAAGGATAGTTCTTACGGCTTCGGATAATTCATAAAAACGAA364420.1309899732031653No Hit
GTGGGTTAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAA362430.13027467204879864No Hit
CCCACTTGGAGTTATATTATTTGACAAACGAGCAAAATACAAATAAAGAT344670.12389087883193836No Hit
ACGGCTTCGGATAATTCATAAAAACGAATTACTATTACTACAAAACAGGA339020.12185999867004309No Hit
CACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAAAAAT338150.12154727906989282No Hit
CGCTGATGTTCCTTTTAATAAAGAAGAAAAATAAATCCGAAGAATCATTT336600.12099013495468261No Hit
TAAGAAAGCACCATGTAGGAACAACTCAAACAGCAAAGAAATAAATCAAT333890.1200160313726054No Hit
CCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGGAA332850.11964220564369016No Hit
CACGATTTTCTACTCATAAAAGGATACTAAAAAGAAGAAGTTCTTTTCTT332740.11960266638390105No Hit
CCCACTTGGAGTTATATTATTAGACAAACGAGCAAAATACAAATAAAGAT330090.11865012967079971No Hit
ATTGATTCAGCGAACCTCTTCTCCAAGTAAATTGATTCCAAGGTTTGCTA329310.11836976037411329No Hit
CCTCACGGTACTTGTTTGCTATCGGTCTCCTACACATATTTAGCTTTATG325610.11703980345393405No Hit
CCACTTATTAATTCAATTAATATGAATACCCGGCGAACCGAGTAATATAT323240.11618791212938684No Hit
CCCCCCTTTAATTGCTGTTCCCTCCTTGTTTCAAGGATAGTTCTTACGGC323170.1161627507822483No Hit
TCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCT322190.11581049192230894No Hit
TCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTC315490.11340219155333577No Hit
CCCTGTGGTAACTTTTCAGACACCTCTATATGCACAGTAGGAAGATGAAT314880.11318292838541433No Hit
CCCCATCACGATATGTACTGATAAAGATTACCTACACTTTTCAGTGGAGG312910.11247481618737296No Hit
GCCACGCTTTCACGTTTTCTACTTTTTCTGAAAATAGAAATCAAACAAGC311280.11188891624686156No Hit
CCAATCTCAAAGAACACGACTCTAGAAGTAAAATTCCAAAAAATAGAAAG311120.11183140459625923No Hit
CTTTTGAGAAGAGTTATAAAACCAGTTGTCCCCTCGTTAGAGTGGGGACT311060.11180983772728335No Hit
CGAGGCAAAAAAGTCGTCTACAGTTTGTGCAATTCTTTTTTGTTGTTAAA309500.11124909913391048No Hit
GCCCCCAAGCTACTCCTATTAATCGTAACTAAGCCAAAATAAGAAAAAAG309120.11111250896372993No Hit
CCCGCTTTACTTTTTTTCTCTCTTAATCCGTTCCATCTTAACGATTTCAG306090.11002338208044801No Hit
AGTCAAACTCCCCACCTGGCTATGTCTCCATAGAGTCGGGAGGAAAGTGA304310.10938356496749692No Hit
GCTCCCTCTCCGGAATCGAACTCTAATTCCCCGTTACCCGTCATAGCCAT303330.10903130610755757No Hit
CTCTTACTCAATATAGCGTAATTGCTTACCGGTATTGTATGCGCCGACAG302370.10868623620394349No Hit
TACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAGTGCTCCAA300520.10802125774385389No Hit
CGGTCTCCTACACATATTTAGCTTTATGTGAAATGTATCACACGAATTAG293950.10565968559099512No Hit
CGGGTCCTACCAAATATGCTCTTACTCAATATAGCGTAATTGCTTACCGG290300.10434770106162912No Hit
ATCTCTATTACACTCAAAATACTATCGGCACAGGAATATCAACCTGTTTC283300.10183156634777654No Hit
CTCGTCACGCTTAGTTCAAAGCCTCTCGGGTCCTACCAAATATGCTCTTA283230.10180640500063803No Hit
AGTTTGTGCAATTCTTTTTTGTTGTTAAAGAACTATTGCCTAGCTCACAG278720.10018529534928443No Hit
CGTTACCCGTCATAGCCATGTTAGGCCAATACCCTAACATCAAAAGCTGA278450.10008824443889297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)