FastQCFastQC Report
Fri 11 Sep 2020
HNTCYDRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTCYDRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038864473
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA19730410.18992284857930647No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG11859380.11415714280567182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG55458000.069.034592
AAGCAGT55613800.068.9913561
GCAGTGG64564900.059.2581863
CAGTGGT71690850.053.3465274
AGTGGTA74773850.051.1558655
GTGGTAT84550050.045.1992156
TGGTATC85584800.044.6164787
GGTATCA87389950.043.7457858
GTATCAA94442700.040.570969
AGAGTAC89847450.023.66740418-19
GCAGAGT90443800.023.60888516-17
TACATGG89712050.023.44193822-23
CAACGCA91698200.023.43772912-13
ACGCAGA92077550.023.31111714-15
ATCAACG93112150.023.09120610-11
CATGGGG40444650.022.891824-25
AGTACAT92624600.022.81281720-21
CATGGGA41057800.021.36420824-25
CAGAGTA90921350.020.99911318-19
CGCAGAG91466300.020.96924816-17
Produced by FastQC (version 0.11.5)