FastQCFastQC Report
Fri 11 Sep 2020
HNTCYDRXX_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTCYDRXX_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038864473
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCTA10808001110.403668024943617No Hit
TCGTCACG982547319.457896920496578No Hit
AGTCGCTT976844649.403003619703144No Hit
CACGAGAA919761778.853529925264853No Hit
TTGTTGAT854912178.229294505867658No Hit
GGCTGAAC814441837.839731275515473No Hit
GTAATTGC810160707.798521569040122No Hit
CAAAGTGG768540357.397888463551299ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
ACACTGTT735155347.076527873525616No Hit
GCTCAACC670407966.453276413082229No Hit
TTTACCCA619169565.960060971302462No Hit
CAGGATGG592668835.70496773547862No Hit
GGGGGGGG204552481.9690006282465298No Hit
AGTCGCGT56101350.5400256862956561No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)