FastQCFastQC Report
Fri 11 Sep 2020
HNTCYDRXX_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNTCYDRXX_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038864473
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA1980300.017.5795541
AGCCGAA1843500.017.2757768
CGGAACC1967950.016.9660131
TACCGGG1929150.016.3468381
GGGCTAC1629900.016.2884621
CCGGACA1748700.016.2124371
GGGGTGA783100.016.1482685
TGCGACG1848300.016.1273821
GGGACCT1564150.016.1067641
CTGGACG1289150.015.7878221
CGGGGTG763600.015.7612824
AGGGAAG1275150.015.7234433
ACGCGAG1122550.015.57488253
CTGAGCG1818600.015.4936491
GGAACCC1723750.015.4872121
CTGTGGG1836900.015.4255961
ATGGGAG1287550.015.1610541
AGGGGTG786900.015.1548154
GGAATGG1976500.015.1537321
TGCGGAG1119150.015.1494553