FastQCFastQC Report
Tue 6 Oct 2020
HNNYJDRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNNYJDRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056376677
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA40070680.37932189220417634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG19362080.18328765128539468No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGTTGAACTCTAGATAA14689340.13905399768684973No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGC12053170.11409916805650945No Hit
GTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGCAAC11179460.10582834933225245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT87669700.076.28491
AGCAGTG94594600.070.629852
GCAGTGG114904150.058.1523173
CAGTGGT126785750.052.651614
AGTGGTA133704450.049.912695
GTGGTAT151288450.044.0916986
TGGTATC152722550.043.6563387
GGTATCA156488400.042.628528
GTATCAA165754450.040.2898459
AGAGTAC160778750.023.61184118-19
GCAGAGT161765350.023.56062516-17
TACATGG160211400.023.48832122-23
CAACGCA163015900.023.48060612-13
ACGCAGA163516550.023.4002414-15
ATCAACG163772000.023.36866410-11
AGTACAT163090150.023.143820-21
CATGGGG71464200.023.06329524-25
CATGGGA70016500.022.56680124-25
TGGGGAG10796150.021.01102626-27
CAGAGTA161681650.020.6144418-19
Produced by FastQC (version 0.11.5)