FastQCFastQC Report
Tue 6 Oct 2020
HNNYJDRXX_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNNYJDRXX_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056376677
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCGAC581888395.508341888543986No Hit
CAGTACTG578576755.476992843529051No Hit
GCAGTAGA560613675.3069485743672855No Hit
CCCACAGT493705364.673573080031187No Hit
CGGTCCCA491370644.651471872660437No Hit
AGTAGTCT482825704.570582733530002No Hit
GGATGCCG474018434.48721029459078No Hit
GATACGCC473783604.484987318590715No Hit
AGCCAATT466896414.419790971965959No Hit
ATGCTGAA465969794.411019290233724No Hit
ACGTCCCT457458814.330451627341258No Hit
GTCCTAAC429230124.063229805669025No Hit
CTAGGTGA423556534.009521785381106RNA PCR Primer, Index 38 (100% over 8bp)
TATGATTC421118313.9864408138575365No Hit
ACAGAGGT421055463.9858458556256067No Hit
GAAGGAAC413022243.9098008219278397No Hit
CGCATGTG404304733.8272780799002817No Hit
TTTCATGA388116813.674038043912626No Hit
GGGGGGGG379695673.5943208352374483No Hit
TATAGTTG320769173.036503711071614No Hit
TTGAGATC260511682.466087009226918No Hit
GCTGTCCA254648812.410587203829378No Hit
CGCTATGT240687502.2784249713229894No Hit
AAACCGAG222568642.106906038782225No Hit
TCGTTCAG95476980.9038156755897404No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)