FastQCFastQC Report
Wed 12 Aug 2020
HNNWMDRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNNWMDRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064177351
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG27304700.064.4771652
AAGCAGT28537200.061.821591
GCAGTGG30705250.057.311863
CAGTGGT33209950.053.028294
AGTGGTA33469550.052.5758555
GTGGTAT35600100.049.4192166
GGTATCA35651750.049.3339048
TGGTATC35693300.049.2466137
GTATCAA42767900.041.390859
CATGGGG20674950.028.12736324-25
AGAGTAC35497650.027.73498218-19
GCAGAGT35918000.027.5143616-17
TACATGG35762450.027.26099422-23
CAACGCA36888500.026.98149312-13
ACGCAGA37361600.026.59530414-15
ATCAACG38793700.025.68751510-11
AGTACAT39112850.025.10297820-21
ACATGGG34954550.024.52580324-25
TCAACGC36141850.024.42501312-13
CAGAGTA36556050.024.03795218-19
Produced by FastQC (version 0.11.5)