FastQCFastQC Report
Wed 12 Aug 2020
HNNWMDRXX_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNNWMDRXX_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064177351
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGCTT511718994.808587492668785No Hit
CCACTACA423083553.9756864737107147No Hit
GTGCCCGT414564613.8956345914563637No Hit
TCAGGGTG401986343.7774374696309434No Hit
ATGAAGAC401570603.7735307899820167No Hit
TTGCCGGA368451533.4623132098589458No Hit
CATATAAC344635933.238519685427885No Hit
GCCATTCC332877403.1280256029429436No Hit
GTACGCGG327588543.078326556115551No Hit
AGCTATCA323436903.0393138859426827No Hit
AGCACACT310585782.9185528117859745No Hit
AGTTGCAG304265842.8591647784467837No Hit
GATTCTGG293158542.754790258639887No Hit
CAAGAATT284207842.6706811579191374No Hit
CCTTTGTC277741002.60991271557329No Hit
TAGGATAA269418092.531702913493035No Hit
TAGTGCGG190636751.791400181754103No Hit
TCGCCAGC183336031.7227958274785724No Hit
TAGGACGT180312111.6943802631258968No Hit
GGCTGTTG174746291.6420786425852059No Hit
TATGAGCT169019931.5882684389136186No Hit
CCGATAGC166264571.5623765140628332No Hit
ACACCTTC161008701.5129874719538172No Hit
GCGGATAG160409391.5073557978777166No Hit
CCTTGTAG159237571.4963442874476287No Hit
CTCGAAAT158419901.4886606997521037No Hit
AGCAGAGC152472861.4327767815836554No Hit
CATCTCCA150574251.4149356764500431No Hit
ATACCCAA149094451.401030099540241No Hit
TTATCGTT147622951.3872025171488545No Hit
GGAATGTC147154301.3827986459373538No Hit
GTCAGCTA145826011.3703167978811832No Hit
ATCCCACA144405251.3569660157144239No Hit
CGATAGCT139458351.3104803430457523No Hit
AGGCCCGA134660481.265395094844487No Hit
GCCGAGAT128260991.2052595357293976No Hit
GGTATGCA127890321.2017763757123976No Hit
CCAAGATG124076191.1659352633600637No Hit
ATGCCTTA115647451.0867309841853605No Hit
TGGAGGCT114150801.072667069006245No Hit
GTTTATCT107357181.0088278978980074No Hit
TAATGACC106434221.0001549074501963No Hit
CGTATCGG106381550.9996599711508048No Hit
CACGTCGG102993720.9678247700274539No Hit
TACGTGAC101631100.9550203253668007RNA PCR Primer, Index 17 (100% over 8bp)
GAATCCGC95581980.8981771685911402No Hit
ACGACATT95235570.8949219780942321No Hit
AATGTTAG95045790.8931386287321952No Hit
TGTCAGAC78881030.7412395116836122No Hit
GTATGTCA77655390.7297222584847137No Hit
CCCGATTA76195810.7160066874980879No Hit
ATTCTAAG68342430.6422090259276717No Hit
GGGGGGGG58819660.5527242235021032No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)