FastQCFastQC Report
Thu 31 Aug 2017
HNNGJAFXX_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNNGJAFXX_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81971024
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT978353011.93535169208085No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC76453379.326877507349426No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGCATCCGCCGTGGGCGGT75740639.239927269909426No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33264554.058086428199311No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGA15574521.900003103535708No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCTTAGACGGC13244331.6157331400422668No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCACAAACCACACCGTGGTAATC12157061.483092366883205No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGACCAA11630821.418894071641706No Hit
GGCTACCTTGTTACGACTTCACCCTAATCATCTGTCCTACCTTAGACGGC11039111.3467088077367437No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCTGCCCTGCCTTAGACGGC10998141.3417107001127617No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTGACCCTACCTTCGGCCGC9378371.1441079472180316No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCTGTCCCGCCTTAGGCGGC9040721.1029165623208512No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGACCAG7994040.9752275389410776No Hit
GGCTACCTTGTTACGACTTCACCCCAATCACCAGTTTTACCTTCGGCGGC6400220.7807905388616347No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCACAGACCACACCGTGGTAATC5890210.7185722115658821No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGA5838870.7123090227590666No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGGCCCTACCGTAGACGGC4135650.5045258431808782No Hit
GGAAACCGTGTTACGACTTTTGCATCCTTTAGAGGGCTCTATTTACGCAA3434890.41903709779201975No Hit
GAAAACCTTGTTACGACTTTTGCCCGGTTCAAACAACAGCGATTGGAAGG2722530.33213321819671304No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAA2435540.2971220659632116No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATTAGGTTTGACTAG2423040.29559713686143535No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC1975320.24097783626565406No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATAATCAAGTTCGGTCAA1913600.23344834633272366No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAG1827180.2229055964946833No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTTGAACAC1607620.1961205218078037No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC1518700.18527278614940812No Hit
AGATACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTGAACTG1301590.15878659756647664No Hit
GGATACCTTGTTACGACTTCTCCTTCCTCCAGGCGCGGGGGCTCGCGGCC1284800.15673831279697079No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA1132600.1381707760537431No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGTCAT1119010.13651287313429195No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTCGAACAC1075560.13121221957651766No Hit
AGATACCTTGTTACGACTTTTGCTTCCTCTAGATGACACGATTCGAGGCA1057500.12900900201027132No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC892860.1089238558249559No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAACGCG871260.10628877833708654No Hit
TGATACCTTGTTACGACTTGTGCTTCCTCTAAATGATACAGTTTGAAGCC831520.10144072373671946No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTTGAGGTA830000.10125529235794346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA48523600.093.944147
CCTTGTT48601550.093.940426
TGTTACG49031500.093.93439
ACCTTGT48525800.093.931365
AAACCTT18542200.093.8320853
GTTACCT19886300.093.8136142
TTGTTAC48558200.093.560818
TACCTTG30143750.093.257244
CCTGAGG2294550.093.1365794
CTCATGG3522250.092.916594
GGCTACC8970250.092.5947951
ACCGTGT622600.092.441465
GAAACCT18563000.092.437062
AACCTTG18899900.092.422994
GCCTTGT186350.092.006775
CCGTGTT632050.092.00656
GATACCT1255050.091.726172
CGTGTTA622300.091.631337
GGAAACC18977450.091.567251
AACCGTG445800.090.488264
Produced by FastQC (version 0.11.2)