FastQCFastQC Report
Thu 31 Aug 2017
HNNGJAFXX_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNNGJAFXX_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81971024
Sequences flagged as poor quality0
Sequence length12
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGG2969876436.23080760830803No Hit
TCGATGAACTCG8000550.9760217220172827No Hit
GCCTCCCTTATG7709440.9405079531518358No Hit
GGTGTCTATTGT7390050.9015441846865303No Hit
TTCTACGGAGCA7160980.8735989439390193No Hit
CGATCCGTATTA7101710.866368340110037No Hit
ATTCCTGTGAGT7076320.863270904118509No Hit
CATCAAACCTCA6989390.8526659371731162No Hit
CTTGCCTTGTGC6578120.8024933298381144No Hit
CCTTATAGGCGA6473820.7897693214128934No Hit
GCTGATGAGCTG6450800.7869610119790623No Hit
GGTAATCCTGTG6412170.7822483710829329No Hit
ACTCTTCTAGAG6398250.7805502100351949No Hit
GTCCGGCTTCTT6358130.7756557975901338No Hit
GATTGCCGCAAG6336740.7730463389111742No Hit
AGATTCGTTGTC6287000.7669783410293862No Hit
GTATGCGCTGTA6093990.7434322157546793No Hit
CCTGTATCCCGT6081980.7419670638736928No Hit
GGATCGCAGATC5991740.7309582957021497No Hit
GACAAGGCTCCA5973330.7287123801210535No Hit
AGCTGTTGTTTG5944730.7252233423361895No Hit
ACAGGAACTCGC5929450.7233592690021782No Hit
TCGAGACGCTTA5928060.7231896968860606No Hit
CCTTTCTTCTCA5888080.7183123636469394No Hit
TCTATCGGGTTA5850460.7137229370222337No Hit
CCACTAATTGAC5707000.6962216307069679No Hit
TTGAAGTCCATC5687580.6938525008544483No Hit
ATAAGGCCTTCT5656200.6900243188373492No Hit
GCTTACATCGAG5543910.6763255757302726No Hit
GCACACACGTTA5530860.6747335497480184No Hit
ACCACCAGAACG5483840.6689973764387767No Hit
GCAATAGCTGCT5424830.6617984911351114No Hit
AAGTGTTTGCCG5393980.6580349661119276No Hit
AGGAGTAACACA5390900.6576592235812498No Hit
TTCAACAGGTGC5379190.6562306699987059No Hit
GATAGTGCCACT5353270.6530685770132627No Hit
CTATTTGCGACA5320000.649009825715975No Hit
ACCTAGAGTCGG5309580.6477386448167343No Hit
GAGTCAACCGCA5252770.6408081470349815No Hit
TGAACTGCCGTA5249180.6403701873969514No Hit
TAATGACCACGC5087590.6206571239124693No Hit
TACACGATCTAC5023520.612840947308405No Hit
GAGGCTCATCAT4988090.6085186882623304No Hit
TCACAGATCCGA4964630.6056567013241166No Hit
ACCGTGCATAGG4905350.598424877551853No Hit
CGAGGCTCAGTA4863560.5933267345787946No Hit
TTACTGTGCGAT4824760.5885933546468811No Hit
CTTGATGCGTAT4798340.5853702644973668No Hit
ATTGCAGGTACC4787250.5840173473982708No Hit
ATGACTCATTCG4786910.5839758693267025No Hit
TGCTCAGTATGT4595360.5606078557710832No Hit
GCGCTAAAGTTC4519280.5513265272860322No Hit
GACAATCTGCTT4509420.5501236632105511No Hit
GTCAAGAACCTC4486070.547275095648433No Hit
TCCGAATTCACA4380070.5343436968653704No Hit
TTAGGGCTCGTA4366020.5326296765549738No Hit
TCGACCAGCAAT4346910.5302983649441784No Hit
CTCAGTATGCAG4343370.5298665050225553No Hit
TGCCAGGCTAGA4336610.5290418233643147No Hit
GGCTCTGCTCTT4283260.5225334259579336No Hit
GCCTTCTAAGGA4245510.5179281400705693No Hit
CATAGTTTAGCC4216580.5143988441574184No Hit
GTGTACCTATCA4133210.5042281770202115No Hit
TCGTTTCGGGAA4124580.5031753659683451No Hit
TAGGATTGCTCG3986130.4862852512370713No Hit
ACAAAGGCCCTG3933320.47984273076788697No Hit
AATGCCTCAACT3825820.46672834049261114No Hit
GACAGCGTTGAC3755880.4581960571823526No Hit
GGAAACCACCAC3690450.450213968292015No Hit
CTACTGATATCG3587120.43760829436509174No Hit
CTAGAGACTCTT3578130.4365115653550943No Hit
GGAGACAAGGGA3533450.4310608587737052No Hit
TGCTCGTAGGAT3520520.4294834721108278No Hit
AGTAGAGGGATG3401490.4149624872320736No Hit
ATTTCGACATGC3392340.41384623912957336No Hit
CACGCCATAATG2807820.34253811444395277No Hit
TACAGATGGCTC2804590.3421440727640538No Hit
AGTTTCTCTAGG2707470.330295983614893No Hit
AGAGGAGACCAA2688560.32798907086972584No Hit
TCTCACTAGGTA2581460.3149234783257069No Hit
TAGAGTACCGGC2311180.28195085131545994No Hit
AATTTGATCCCG2263110.2760865839616692No Hit
ATGGCAGCTCTA2240470.273324632372532No Hit
CGATGTCGTCAA2205200.26902189241896013No Hit
AGGATCCGCATG2134220.26036273500743384No Hit
CCTCGTTCGACT2057970.25106066748659867No Hit
CAGGCGTATTGG2041400.2490392214692841No Hit
CGGAGCTATGGT2032870.24799860985023195No Hit
GAATCTTCGAGC1967190.2399860223778588No Hit
TCTAGCGTAGTG1929510.23538927609346444No Hit
CGCAGCGGTATA1875390.22878694305441394No Hit
CCAGTGTATGCA1854760.22627020006484241No Hit
TGGCTCTACAGA1826440.22281532069185814No Hit
CGACTCCAAGAT1819670.22198941909033612No Hit
TAGGAACTGGCC1816730.22163075576559832No Hit
AAGGAGCGCCTT1814110.22131113062586602No Hit
ACTGTCGAAGCT1752870.21384019797044382No Hit
CGTGTTTCAAAC1695510.2068426033082129No Hit
ACGTGTACCCAA1622710.19796141621946797No Hit
TGAGTCACTGGT1591260.19412469459939893No Hit
GGAGTCGGTCTA1543980.1883568027648404No Hit
TCCTCTGTCGAC1513920.18468965326088888No Hit
CTAGCGAACATC1499390.18291707567298415No Hit
AGAGTCCTGAGC1499210.18289511669391856No Hit
GATCTATCCGAG1490710.1818581649047107No Hit
ACGTTAGCACAC1429000.1743298949150617No Hit
CCTACGCCTCTA1426090.17397489142016817No Hit
TCGAGGACTGCA1260370.15375799136045926No Hit
GCACCCAAAGTG1254200.15300528635582253No Hit
ACCTGTCTCTCT1240770.1513669025288741No Hit
ATGGGCTCTGAT1102490.13449752683338445No Hit
GAGTGGTAGAGA1070660.13061444736862138No Hit
TAGCTCGTAACT1066540.13011183073667593No Hit
CTGCATAGATCT1054490.12864179908256362No Hit
TTGCCACTAGAT1008290.12300566112239857No Hit
CTAACCTCCGCT902180.11006084296324029No Hit
TCCCATTGAAGG862540.10522498779568742No Hit
CAGTAACGGCCA821830.1002585986970225No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCT550.0091389376.0014541
GCACGCA550.0091389376.0014541
TCTAGCA600.0033878836.0014541
CCATCCG909.1194925E-66.0014541
CTTGCTG1001.2815544E-66.0014541
GCTTACT806.518073E-56.0014541
ACGTGGA600.0033878836.0014541
ACCGTCC751.7459592E-46.0014541
ACCGTAC909.1194925E-66.0014541
CCAGTGT188200.06.0014541
CATTTCT1054.811045E-76.0014541
AGCAGTT1351.36788E-96.0014541
CCTTACT650.00125851966.00145341
TTCACGG2500.06.00145341
TCCTCGT650.00125851966.00145341
GCTGACA704.6837144E-46.00145341
TCGATCA704.6837144E-46.00145341
ACTCGTC1405.16593E-106.00145341
AGACAGC6950.06.00101042
CGTATCG1101.81024E-76.001012
Produced by FastQC (version 0.11.2)