FastQCFastQC Report
Thu 31 Aug 2017
HNNGJAFXX_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNNGJAFXX_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81971024
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG77625179.469830461066339No Hit
ACACCATGGGAGTTGTATTCGCCTTAAGTCGGGATACTAAATTGGTTACC66401328.100584421148623No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGG61613387.516482897663936No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGTCCTCGGATCGGCCCCGGCGG36279304.425868828965709No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34786394.243742276539085No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGGG14713231.7949306086501982No Hit
ACACCATGAGAGTTTGTAACACCCAAAGCCGGTGGGGTAACCTTTTGGAG11218741.368622648900909No Hit
ACACCATGAGAGTCTGTAACACCCAAAGCCGGTGGGATAACCTTTATAGG10050861.2261479129503128No Hit
ACACCATGGGAGTTTGTAACGCCCAAAGTCGGTGGCCTAACCTTTATGGA8959311.092985004066803No Hit
ACACCATGGGAGTTGTATTCGCCTTAAGTCGGAATGCCAAACTGGCTACC7821350.9541603384142182No Hit
ACACCATGGGAGTCTGCAATGCCCAAAGCCGGTGGCCTAACCTTCGGGAA7436770.9072437596973291No Hit
ACACCATGAGAGTTGGCAATACCCGAAGTCCGTGAGCTAACCGCAAGGAG7014440.8557218950930758No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAAGA6967060.8499418038257032No Hit
ACACCATGGGAGCGGATTGCACCAGAAGTAGTTAGCTTAACCGAAAGGAG5873440.7165263666829391No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCAGCTTAGAGAA5470380.667355332781984No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATGCGCTTAGCCTG5431890.6626597710917945No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCCGCAAGGG5208550.6354135578445379No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATTGCGGCGACGTG3842790.46879858424118254No Hit
ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAG3729650.45499614595518534No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCTGAAAGGG3682300.44921971451765685No Hit
ACACCACGAAAGTCGGTTTTACCCGAAGCCGGTGAGCCAACTAGCAATAG3049040.37196558627839027No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCTGCAAAGG3027250.3693073298681739No Hit
GCTGCCCGGAACTGAGCAATATCGAGAGGCAGGAAGAGATATAAATAATT2838620.34629553975072946No Hit
GCTACTACCGATTGGATGGCTTAGTGAGGCCCTCGGATCGGCCCCGCCGG2804290.3421074744656112No Hit
ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGTAAGGAG2627850.320582795208219No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTGGGATTGGCTTAGGAGG2457660.29982058050171484No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGTG2393830.29203368253640455No Hit
GCTACTACCGATTGAATGGTTTGGTGAGGCTTCCGGATTGACCTAAGAAC2360470.2879639517495841No Hit
ACACCATGAGAGTTGGCAATACCCGAAGTCCGTAAGCTAACCGTAAGGAG2342590.28578269316240335No Hit
GCTACTACCGATTGAATTATTTAGTGAGGTCTCCGGACGTGATCACTGTA1974410.24086682142704474No Hit
ACACCATGGAAGTCTGCAATGCCCGAAGCCGGTGGCCTAACCACTTATGT1962360.23939678977293244No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTCCGGATTGGTTTAGAGAA1648240.2010759314169358No Hit
GCTGCAACCGATCGGAGGGTCCTGTGAATTCATCGGATTGGCCTACTTCG1623750.19808829032073577No Hit
AAACCATCCGAGTTGGGTTTCAGTGAGGTTGCCTCTGATTAGGGTGTTCA1530490.18671109927820348No Hit
ACACCATGAGAGTTTGTAACACCCAAAGCCGGTGGAGTAACCATTTGGAG1413320.17241702384979354No Hit
GCTGTCCGGGACTGAGCTGTCTCGAGAGGACTGCGGACTGCTGTATCGAG1356290.16545968731584956No Hit
ACACCATGGGAGTCTGCAATGCCCGAAGCCGGTGGCCTAACCACTATGGA1303340.15900008764072535No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA1283310.15655654124803906No Hit
GCTCCTACCGACTGGGCGCGGCGGCGAGCGTCCCGGACCCGCGAAGGGCC1222010.1490782889329283No Hit
GCACCATGGGAGTTGTATTCGCCTTAAGTCGGGATACTAAATTGGTTACC1201100.14652738753147695No Hit
GCACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG1196780.14600037203390312No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG1187630.14488412393140288No Hit
GCTGTTACCGATTGGATGGCTCAATGAGTAGCAAGGAGGTTTGTTGGGTC1078420.13156112335500408No Hit
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACTGGCTAGCTTGG1047610.12780247810494597No Hit
AAACCATCCGAGTTGGGTTTCAGTGAGGTCACCTCTAATTAGGGTGTTCG1022920.12479043814311751No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACTGCTGTTTCTCA893850.10904463020981658No Hit
ACACCACGAAAGTCGGTTTTACCCGAAGCCGGCGAGCCAACCAGCAATGG884840.10794546131325626No Hit
GTTGTTACCGATTGAATGGTTTAGTGAGTATTCGGGAGGGGGTATCTGCG821000.10015734340466456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC2285800.093.941822
ACCATGG23126000.093.919093
CCATGGG22979550.093.91824
CTACTAC13041300.093.915232
CCGATTG18817900.093.9122248
ATGGGAG22968600.093.864236
ACTACCG13025850.093.854974
TGGGAGT20777900.093.845857
TACTACC13029200.093.834873
CATGGGA22988650.093.726775
GGGAGTT10312700.093.707498
AGTACCG2471200.093.693444
CCATGAG5252900.093.665134
CATGAGA5241800.093.649095
CTCCTAC2960550.093.6446462
CGATTGA7219250.093.636369
ATGAGAG5255600.093.609116
GCTAGTA2282200.093.6006241
GGGAGTG9466150.093.561438
GAGAGTT3198450.093.4935468
Produced by FastQC (version 0.11.2)