FastQCFastQC Report
Sat 3 Oct 2020
HNMLVDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNMLVDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58393294
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC346050.022.5068288
CGTCTGA213650.017.52773316-17
CGGAAGA482250.017.0464954
ACGTCTG220750.017.02828614-15
GCCCCCC49000.016.685721
TCGGAAG498500.016.3584883
CACGTCT233900.016.16225814-15
ACACGTC238650.015.88034412-13
ATCGGAA520550.015.5602842
GATCGGA536150.015.0810171
CTGAACT254750.014.82127818-19
CTCCCGT23200.014.7522121
GAACTCC249350.014.7096920-21
CACACGT260000.014.66759812-13
TGAACTC260400.014.35899320-21
GCACACG277350.013.792768510-11
AACTCCA271900.013.57971322-23
GTCACGA35700.013.56649828-29
CTCCAGT260150.013.017324-25
TGAACCG17650.012.90752512-13
Produced by FastQC (version 0.11.5)